Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics

Abstract EN

Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes.

Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms.

It usually affects immunocompromised individuals but can also infect immunocompetent individuals.

There is no vaccine against it available till now.

This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools.

The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells.

Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests.

Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set.

Nineteen epitopes were shared between MHC I and II results.

For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles.

We recommend in vivo and in vitro studies to prove its effectiveness.