Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics
Joint Authors
Elhag, Mustafa
Alaagib, Ruaa Mohamed
Ahmed, Nagla Mohamed
Abubaker, Mustafa
Haroun, Esraa Musa
Albagi, Sahar Obi Abd
Hassan, Mohammed A.
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes.
Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms.
It usually affects immunocompromised individuals but can also infect immunocompetent individuals.
There is no vaccine against it available till now.
This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools.
The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells.
Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests.
Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set.
Nineteen epitopes were shared between MHC I and II results.
For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles.
We recommend in vivo and in vitro studies to prove its effectiveness.
American Psychological Association (APA)
Elhag, Mustafa& Alaagib, Ruaa Mohamed& Ahmed, Nagla Mohamed& Abubaker, Mustafa& Haroun, Esraa Musa& Albagi, Sahar Obi Abd…[et al.]. 2020. Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1187698
Modern Language Association (MLA)
Elhag, Mustafa…[et al.]. Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics. Journal of Immunology Research No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1187698
American Medical Association (AMA)
Elhag, Mustafa& Alaagib, Ruaa Mohamed& Ahmed, Nagla Mohamed& Abubaker, Mustafa& Haroun, Esraa Musa& Albagi, Sahar Obi Abd…[et al.]. Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1187698
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187698