Expression Profiling of Long Noncoding RNA Splice Variants in Human Microvascular Endothelial Cells: Lipopolysaccharide Effects In Vitro
Joint Authors
Chowdhury, Imran H.
Narra, Hema P.
Sahni, Abha
Khanipov, Kamil
Schroeder, Casey L. C.
Patel, Jignesh
Fofanov, Yuriy
Sahni, Sanjeev K.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-25
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Endothelial cell interactions with lipopolysaccharide (LPS) involve both activating and repressing signals resulting in pronounced alterations in their transcriptome and proteome.
Noncoding RNAs are now appreciated as posttranscriptional and translational regulators of cellular signaling and responses, but their expression status and roles during endothelial interactions with LPS are not well understood.
We report on the expression profile of long noncoding (lnc) RNAs of human microvascular endothelial cells in response to LPS.
We have identified a total of 10,781 and 8310 lncRNA transcripts displaying either positive or negative regulation of expression, respectively, at 3 and 24 h posttreatment.
A majority of LPS-induced lncRNAs are multiexonic and distributed across the genome as evidenced by their presence on all chromosomes.
Present among these are a total of 44 lncRNAs with known regulatory functions, of which 41 multiexonic lncRNAs have multiple splice variants.
We have further validated splice variant-specific expression of EGO (NONHSAT087634) and HOTAIRM1 (NONHSAT119666) at 3 h and significant upregulation of lnc-IL7R at 24 h.
This study illustrates the genome-wide regulation of endothelial lncRNA splice variants in response to LPS and provides a foundation for further investigations of differentially expressed lncRNA transcripts in endothelial responses to LPS and pathophysiology of sepsis/septic shock.
American Psychological Association (APA)
Chowdhury, Imran H.& Narra, Hema P.& Sahni, Abha& Khanipov, Kamil& Schroeder, Casey L. C.& Patel, Jignesh…[et al.]. 2017. Expression Profiling of Long Noncoding RNA Splice Variants in Human Microvascular Endothelial Cells: Lipopolysaccharide Effects In Vitro. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1188247
Modern Language Association (MLA)
Chowdhury, Imran H.…[et al.]. Expression Profiling of Long Noncoding RNA Splice Variants in Human Microvascular Endothelial Cells: Lipopolysaccharide Effects In Vitro. Mediators of Inflammation No. 2017 (2017), pp.1-18.
https://search.emarefa.net/detail/BIM-1188247
American Medical Association (AMA)
Chowdhury, Imran H.& Narra, Hema P.& Sahni, Abha& Khanipov, Kamil& Schroeder, Casey L. C.& Patel, Jignesh…[et al.]. Expression Profiling of Long Noncoding RNA Splice Variants in Human Microvascular Endothelial Cells: Lipopolysaccharide Effects In Vitro. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1188247
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188247