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Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes
Joint Authors
Pufe, T.
Lippross, Sebastian
Tohidnezhad, Mersedeh
Jahr, Holger
Cremer, Jochen
Bayer, Andreas
Lammel, Justus
Behrendt, Peter
Klüter, Tim
Rademacher, Franziska
Gläser, Regine
Harder, Jürgen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Autologous thrombocyte concentrate lysates, for example, platelet-released growth factors, (PRGFs) or their clinically related formulations (e.g., Vivostat PRF®) came recently into the physicians’ focus as they revealed promising effects in regenerative and reparative medicine such as the support of healing of chronic wounds.
To elucidate the underlying mechanisms, we analyzed the influence of PRGF and Vivostat PRF on human keratinocyte differentiation in vitro and on epidermal differentiation status of skin wounds in vivo.
Therefore, we investigated the expression of early (keratin 1 and keratin 10) and late (transglutaminase-1 and involucrin) differentiation markers.
PRGF treatment of primary human keratinocytes decreased keratin 1 and keratin 10 gene expression but induced involucrin and transglutaminase-1 gene expression in an epidermal growth factor receptor- (EGFR-) dependent manner.
In concordance with these results, microscopic analyses revealed that PRGF-treated human keratinocytes displayed morphological features typical of keratinocytes undergoing terminal differentiation.
In vivo treatment of artificial human wounds with Vivostat PRF revealed a significant induction of involucrin and transglutaminase-1 gene expression.
Together, our results indicate that PRGF and Vivostat PRF induce terminal differentiation of primary human keratinocytes.
This potential mechanism may contribute to the observed beneficial effects in the treatment of hard-to-heal wounds with autologous thrombocyte concentrate lysates in vivo.
American Psychological Association (APA)
Bayer, Andreas& Tohidnezhad, Mersedeh& Lammel, Justus& Lippross, Sebastian& Behrendt, Peter& Klüter, Tim…[et al.]. 2017. Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188475
Modern Language Association (MLA)
Bayer, Andreas…[et al.]. Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes. Mediators of Inflammation No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1188475
American Medical Association (AMA)
Bayer, Andreas& Tohidnezhad, Mersedeh& Lammel, Justus& Lippross, Sebastian& Behrendt, Peter& Klüter, Tim…[et al.]. Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188475