Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4+CD25+ Regulatory T Cells Mainly through Axl Receptor
Joint Authors
Dong, Ning
Yao, Yong-Ming
Lu, Zhongqiu
Zhao, Guang-Ju
Hong, Guang-Liang
Zheng, Jia-yi
Bian, Jia-lan
Chen, Long-wang
Yu, Yan
Chandoo, Arvine
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Growth arrest-specific (Gas) 6 is one of the endogenous ligands of TAM receptors (Tyro3, Axl, and Mertk), and its role as an immune modulator has been recently emphasized.
Naturally occurring CD4+CD25+ regulatory T cells (Tregs) are essential for the active suppression of autoimmunity.
The present study was designed to investigate whether Tregs express TAM receptors and the potential role of Gas6-TAM signal in regulating the suppressive function of Tregs.
Methods.
The protein and mRNA levels of TAM receptors were determined by using Western blot, immunofluorescence, flow cytometry, and RT-PCR.
Then, TAM receptors were silenced using targeted siRNA or blocked with specific antibody.
The suppressive function of Tregs was assessed by using a CFSE-based T cell proliferation assay.
Flow cytometry was used to determine the expression of Foxp3 and CTLA4 whereas cytokines secretion levels were measured by ELISA assay.
Results.
Tregs express both Axl and Mertk receptors.
Gas6 increases the suppressive function of Tregs in vitro and in mice.
Both Foxp3 and CTLA-4 expression on Tregs are enhanced after Gas6 stimulation.
Gas6 enhances the suppressive activity of Tregs mainly through Axl receptor.
Conclusion.
Gas6 has a direct effect on the functions of CD4+CD25+Tregs mainly through its interaction with Axl receptor.
American Psychological Association (APA)
Zhao, Guang-Ju& Zheng, Jia-yi& Bian, Jia-lan& Chen, Long-wang& Dong, Ning& Yu, Yan…[et al.]. 2017. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4+CD25+ Regulatory T Cells Mainly through Axl Receptor. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188612
Modern Language Association (MLA)
Zhao, Guang-Ju…[et al.]. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4+CD25+ Regulatory T Cells Mainly through Axl Receptor. Mediators of Inflammation No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1188612
American Medical Association (AMA)
Zhao, Guang-Ju& Zheng, Jia-yi& Bian, Jia-lan& Chen, Long-wang& Dong, Ning& Yu, Yan…[et al.]. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4+CD25+ Regulatory T Cells Mainly through Axl Receptor. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188612