Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells
Joint Authors
Leite, Jacqueline Alves
Araújo, Demetrius A. M.
Rodrigues-Mascarenhas, Sandra
Faheina-Martins, Glaucia V.
Dantas, Bruna Braga
Lima-Júnior, Cláudio G.
Vasconcellos, Mário L. A. A.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers.
The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium falciparum, Leishmania amazonensis, and Leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related.
However, little is known about the mechanisms induced by MBHA in inflammatory process and its relation with anticancer activity.
The present work investigated the cytotoxicity of three MBHA derivatives (A2CN, A3CN, and A4CN), on human colorectal adenocarcinoma, HT-29 cells, and their anti-inflammatory activities were examined in lipopolysaccharide- (LPS-) stimulated RAW264.7 macrophage cells, being these derivatives potentially cytotoxic to HT-29 cells.
Coincubation with A2CN, A3CN, or A4CN and LPS in RAW264.7 cells inhibited NO production, as well as the production of reactive oxygen species (ROS) was also repressed.
The mRNA expressions of IL-1β and IL-6 were significantly downregulated by such MBHA compounds in RAW264.7 cells, but only A2CN was able to inhibit the COX-2 gene expression.
We also showed that MBHA compounds decreased almost to zero the production of IL-1β and IL-6.
These findings display that such MBHA compounds exhibit anticancer and anti-inflammatory activities.
American Psychological Association (APA)
Faheina-Martins, Glaucia V.& Leite, Jacqueline Alves& Dantas, Bruna Braga& Lima-Júnior, Cláudio G.& Vasconcellos, Mário L. A. A.& Rodrigues-Mascarenhas, Sandra…[et al.]. 2017. Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188622
Modern Language Association (MLA)
Faheina-Martins, Glaucia V.…[et al.]. Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188622
American Medical Association (AMA)
Faheina-Martins, Glaucia V.& Leite, Jacqueline Alves& Dantas, Bruna Braga& Lima-Júnior, Cláudio G.& Vasconcellos, Mário L. A. A.& Rodrigues-Mascarenhas, Sandra…[et al.]. Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188622
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188622