The Performance of Immunocytochemistry Staining as Triaging Tests for High-Risk HPV-Positive Women: A 24-Month Prospective Study
Joint Authors
Li, Yu-Cong
Zhao, Yu-Qian
Li, Ting-Yuan
Chen, Wen
Liao, Guang-Dong
Wang, Hai-Rui
Lei, Hai-Ke
Guo, Yue
Zhou, Qi
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
It is urgent to develop an accurate approach to improve the predictive performance of hrHPV-based screening.
The aim is to evaluate the performance of p16/Ki-67 and p16/MCM2 staining to triage high-risk human papillomavirus- (hrHPV-) positive women.
Cervical specimens were collected from eligible women and tested for hrHPV genotyping, cytology, p16/Ki-67, and p16/MCM2 staining at baseline.
Women were invited to participate in follow-up screening by cytology and hrHPV testing at 24 months.
Positive women received colposcopy and biopsies.
Histopathological diagnoses were the gold standard.
485 women came back for the follow-up screening.
The positive rate of p16/Ki-67 was 20.2% and of p16/MCM2 was 27.2%.
The positive rates of p16/Ki-67 ( P<0.001) and p16/MCM2 (P=0.021) were increased by the severity of histopathology findings.
Among hrHPV-positive women, the sensitivity, specificity, PPV, and NPV for p16/Ki-67 were 90.9%, 67.0%, 16.5%, and 99.0%, and for p16/MCM2 were 81.8%, 43.1%, 9.4%, and 97.1%.
The sensitivity of cytology for triaging hrHPV-positive women were lower than p16/Ki-67 (P=0.012) and p16/MCM2 (P=0.065).
The cocktail staining did not add sensitivity to p16/Ki-67 or p16/MCM2 staining alone (P>0.05), however, cutting down the specificity of p16/Ki-67 staining alone with statistical significance (67.0% vs.
40.2%, P<0.001).
The risk of CIN2+ within 24 months for hrHPV-positive but triaging negative women at baseline was 0.5 (0.1–2.7), 0.7 (0.1–4.1), and 2.4 (1.1–5.0) for p16/Ki-67, p16/MCM2, and cytology, respectively.
As an objective and accurate immunocytochemical staining, the p16/Ki-67 and p16/MCM2 dual staining performed better than cytology to triage positive hrHPV.
On condition that high-quality cytology is unavailable, immunocytochemical staining by p16/Ki-67 or p16/MCM2 is an option for triaging hrHPV-positive women.
The combination of p16/Ki-67 and p16/MCM2 could not improve the accuracy in detecting CIN2+.
American Psychological Association (APA)
Li, Yu-Cong& Zhao, Yu-Qian& Li, Ting-Yuan& Chen, Wen& Liao, Guang-Dong& Wang, Hai-Rui…[et al.]. 2020. The Performance of Immunocytochemistry Staining as Triaging Tests for High-Risk HPV-Positive Women: A 24-Month Prospective Study. Journal of Oncology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1189058
Modern Language Association (MLA)
Li, Yu-Cong…[et al.]. The Performance of Immunocytochemistry Staining as Triaging Tests for High-Risk HPV-Positive Women: A 24-Month Prospective Study. Journal of Oncology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1189058
American Medical Association (AMA)
Li, Yu-Cong& Zhao, Yu-Qian& Li, Ting-Yuan& Chen, Wen& Liao, Guang-Dong& Wang, Hai-Rui…[et al.]. The Performance of Immunocytochemistry Staining as Triaging Tests for High-Risk HPV-Positive Women: A 24-Month Prospective Study. Journal of Oncology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1189058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1189058