Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes
Joint Authors
Yu, Xiaoting
Lin, Ping
Ren, Qian
Wu, Jiali
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
This research is aimed at determining the vascular health characteristics of carotenoids by evaluating their effect on excessive inflammatory response in endothelial and monocyte cells, the main factors of atherosclerosis.
Methods.
Human umbilical vein endothelial cells (HUVECs) or U937 monocytes were treated with escalating concentrations (0.1, 0.5, and 1 μM) of five most common carotenoids in human plasma, i.e., α-carotene, β-carotene, β-cryptoxanthin, lutein, and lycopene prior to stimulation with 2 mM fructose.
We examined the monocyte adhesion to endothelial cells (ECs) and relevant endothelial adhesion molecules.
Chemokine and proinflammatory cytokine production as well as intracellular oxidative stress were also assessed in fructose-stimulated ECs and monocytes.
Results.
Carotenoids repressed monocyte adhesion to fructose-stimulated ECs dose dependently via decreasing primarily the expression of endothelial VCAM-1.
In ECs and monocytes, three carotenoids, i.e., β-cryptoxanthin, lutein, and lycopene, suppressed the fructose-induced expression of chemokines MCP-1, M-CSF, and CXCL-10 and inflammatory cytokines TNF-α and IL-1β, with CXCL-10 being the most repressed inflammatory mediator.
β-Cryptoxanthin, lutein, and lycopene dramatically downregulated the fructose-induced CXCL-10 expression in vascular cells.
The reduction in the inflammatory response was associated with a slight but significant decrease of intracellular oxidative stress.
Conclusions.
Our results show that carotenoids have a variety of anti-inflammatory and antiatherosclerosis activities, which can help prevent or reduce fructose-induced inflammatory vascular diseases.
American Psychological Association (APA)
Lin, Ping& Ren, Qian& Yu, Xiaoting& Wu, Jiali. 2020. Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191816
Modern Language Association (MLA)
Lin, Ping…[et al.]. Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes. Mediators of Inflammation No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1191816
American Medical Association (AMA)
Lin, Ping& Ren, Qian& Yu, Xiaoting& Wu, Jiali. Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191816