Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines
Joint Authors
Chen, Chien-Lin
Lei, Wei-Yi
Hsiung, Shih-Chieh
Wen, Shao-Hsuan
Hsieh, Chin-Hsuan
Chang, Chien-Chung
Liao, Shuen-Kuei
Wallace, Christopher Glenn
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC).
Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were newly established in this study.
Among the four, sHCC29 showed the highest growth rate in vitro and tumorigenicity in NOD-SCID mice.
Unlike sHCC74 and SAR-HCV, both sHCC29 and sHCC63 had no detectable surface HLA class I antigen expression, alongside undetected intracellular β2-microglobulin (β2m) and marked HLA class I heavy chain and selective antigen-processing machinery (APM) component downregulation.
The loss of β2m in sHCC29 and sHCC63 was caused by a >49 kb deletion across the B2M locus, while their downregulation of APM components was transcriptional, reversible by IFN-γ only in several components.
β2m was also undetected in the primary HCC lesions of the patients involved, indicating its in vivo relevance.
We report for the first time HLA class I antigen loss with underlying B2M gene deficiency and APM defects in 50% (2 of 4) of the sHCC cell lines tested.
These findings may have implications for a proper design of T cell immunotherapy for the treatment of sHCC patients.
American Psychological Association (APA)
Lei, Wei-Yi& Hsiung, Shih-Chieh& Wen, Shao-Hsuan& Hsieh, Chin-Hsuan& Chen, Chien-Lin& Wallace, Christopher Glenn…[et al.]. 2018. Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193092
Modern Language Association (MLA)
Lei, Wei-Yi…[et al.]. Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1193092
American Medical Association (AMA)
Lei, Wei-Yi& Hsiung, Shih-Chieh& Wen, Shao-Hsuan& Hsieh, Chin-Hsuan& Chen, Chien-Lin& Wallace, Christopher Glenn…[et al.]. Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193092
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193092