Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses
Joint Authors
Wang, Changquan
Zhong, Zhenmin
Sun, Peng
Zhong, Hanbing
Li, Hongzhe
Chen, Fangyi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades.
The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their function.
Startle response is a behavior closely related to the auditory function of hair cells; therefore it can be used to measure the function of hair cells.
In this study, we developed a device to measure the startle response of zebrafish larvae.
By applying various levels of stimulus, it showed that the system can discern a 10 dB difference.
The hair cell in zebrafish can regenerate after damage due to noise exposure or drug treatment.
With this device, we measured the startle response of zebrafish larvae during and after drug treatment.
The results show a similar trend to the classical hair cell staining method.
The startle response was reduced with drug treatment and recovered after removal of the drug.
Together it demonstrated the capability of this behavioral assay in evaluating the hair cell functions of fish larvae and its potential as a high-throughput screening tool for auditory-related gene and drug discovery.
American Psychological Association (APA)
Wang, Changquan& Zhong, Zhenmin& Sun, Peng& Zhong, Hanbing& Li, Hongzhe& Chen, Fangyi. 2017. Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses. Neural Plasticity،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1193254
Modern Language Association (MLA)
Wang, Changquan…[et al.]. Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses. Neural Plasticity No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1193254
American Medical Association (AMA)
Wang, Changquan& Zhong, Zhenmin& Sun, Peng& Zhong, Hanbing& Li, Hongzhe& Chen, Fangyi. Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1193254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193254