Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice
Joint Authors
Lagali, Neil
Morikawa, Tetsuro
Yoshida, Koki
Sato, Jun
Harada, Fumiya
Lennikov, Anton
Mukwaya, Anthony
Schaupper, Mira
Uehara, Osamu
Takai, Rie
Horie, Yukihiro
Sakaguchi, Hiroyuki
Wu, Ching-Zong
Kitaichi, Nobuyoshi
Abiko, Yoshihiro
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Purpose.
Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect.
Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects.
The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice.
Methods.
C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm2).
The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL.
NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR.
Results.
Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p<0.01), with significantly less NF-κB nucleus translocation (p<0.001), and significantly fewer TUNEL cells (p<0.01).
Weaker DHE signals were detected in the nano-AST group (p<0.05) relative to the others.
Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45.
Conclusions.
Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity.
American Psychological Association (APA)
Harada, Fumiya& Morikawa, Tetsuro& Lennikov, Anton& Mukwaya, Anthony& Schaupper, Mira& Uehara, Osamu…[et al.]. 2017. Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193973
Modern Language Association (MLA)
Harada, Fumiya…[et al.]. Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1193973
American Medical Association (AMA)
Harada, Fumiya& Morikawa, Tetsuro& Lennikov, Anton& Mukwaya, Anthony& Schaupper, Mira& Uehara, Osamu…[et al.]. Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193973