The Neuroprotective Effects of SIRT1 on NMDA-Induced Excitotoxicity
Joint Authors
Yan, Liang-Jun
Zhang, Ce
Yang, Xiaorong
Si, Peipei
Qin, Huaping
Yin, Litian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Silent information regulator 1 (SIRT1), an NAD+-dependent deacetylase, is involved in the regulation of gene transcription, energy metabolism, and cellular aging and has become an important therapeutic target across a range of diseases.
Recent research has demonstrated that SIRT1 possesses neuroprotective effects; however, it is unknown whether it protects neurons from NMDA-mediated neurotoxicity.
In the present study, by activation of SIRT1 using resveratrol (RSV) in cultured cortical neurons or by overexpression of SIRT1 in SH-SY5Y cell, we aimed to evaluate the roles of SIRT1 in NMDA-induced excitotoxicity.
Our results showed that RSV or overexpression of SIRT1 elicited inhibitory effects on NMDA-induced excitotoxicity including a decrease in cell viability, an increase in lactate dehydrogenase (LDH) release, and a decrease in the number of living cells as measured by CCK-8 assay, LDH test, and Calcein-AM and PI double staining.
RSV or overexpression of SIRT1 significantly improved SIRT1 deacetylase activity in the excitotoxicity model.
Further study suggests that overexpression of SIRT1 partly suppressed an NMDA-induced increase in p53 acetylation.
These results indicate that SIRT1 activation by either RSV or overexpression of SIRT1 can exert neuroprotective effects partly by inhibiting p53 acetylation in NMDA-induced neurotoxicity.
American Psychological Association (APA)
Yang, Xiaorong& Si, Peipei& Qin, Huaping& Yin, Litian& Yan, Liang-Jun& Zhang, Ce. 2017. The Neuroprotective Effects of SIRT1 on NMDA-Induced Excitotoxicity. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194173
Modern Language Association (MLA)
Yang, Xiaorong…[et al.]. The Neuroprotective Effects of SIRT1 on NMDA-Induced Excitotoxicity. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1194173
American Medical Association (AMA)
Yang, Xiaorong& Si, Peipei& Qin, Huaping& Yin, Litian& Yan, Liang-Jun& Zhang, Ce. The Neuroprotective Effects of SIRT1 on NMDA-Induced Excitotoxicity. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194173