Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation
Joint Authors
Zhang, Liangqing
Feng, Du
Wang, Shuang
Deng, Fan
Cai, Shuyun
Xu, Riping
Wang, Jingjing
Hu, Zhe
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Endothelial microvesicles (EMVs), released after endothelial cell (EC) apoptosis or activation, may carry many adverse signals and propagate injury by intercellular transmission.
Caveolae are 50–100 nm cell surface plasma membrane invaginations involved in many pathophysiological processes.
Recent evidence has indicated EMVs and caveolae may have functional effects in cells undergoing H/R injury.
Propofol, a widely used anaesthetic, confers antioxidative stress capability in the same process.
But the connection between EMVs, H/R, and caveolae remains largely unclear.
Here, we found that H/R significantly increased the release of EMVs, the expression of CAV-1 (the structural protein responsible for maintaining the shape of caveolae), oxidative stress, and the mitochondrial damage, and all these changes were inhibited by propofol preconditioning.
Interestingly, the caveolae inhibitor Mβ-CD strengthened the protective effect of propofol preconditioning.
We further found that the release of EMVs is more significantly reduced under propofol preconditioning in the presence of the caveolae inhibitor Mβ-CD.
EMVs released from H/R-treated cells caused a substantially increased mitochondrial and cellular damage to normal HUVECs after 4 hours of coculture.
Thus, we conclude that inhibition of caveolae contributes to propofol preconditioning-suppressed microvesicles release and cell injury by H/R.
American Psychological Association (APA)
Deng, Fan& Wang, Shuang& Cai, Shuyun& Hu, Zhe& Xu, Riping& Wang, Jingjing…[et al.]. 2017. Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1194369
Modern Language Association (MLA)
Deng, Fan…[et al.]. Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1194369
American Medical Association (AMA)
Deng, Fan& Wang, Shuang& Cai, Shuyun& Hu, Zhe& Xu, Riping& Wang, Jingjing…[et al.]. Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1194369
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194369