Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils
Joint Authors
Sun, Yihua
Zhai, Tai-yu
Cui, Bao-hong
Zou, Lei
Zeng, Jing-ya
Gao, Song
Zhao, Qianyu
Wang, Yuan
Xie, Wan-lin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The calcium-sensing receptors (CaSRs) play an important role in many tissues and organs that are involved in inflammatory reactions.
Peripheral blood polymorphonuclear neutrophils (PMNs) are important inflammatory cells.
However, the expression and functions of CaSR in peripheral blood PMNs are still not reported.
In this study, we collected rat peripheral blood PMNs to observe the relationship between CaSR and PMNs.
From the results, we found first that the CaSR protein was expressed in PMNs, and it increased after PMNs were activated with fMLP.
In addition, CaSR activator cincalcet promoted the expression of CaSR and P-p65 (NF-κB signaling pathway protein) and Bcl-xl (antiapoptosis protein), and it increased the secretion of interleukin-6 (IL-6) and myeloperoxidase (MPO); meanwhile, it decreased proapoptosis protein Bax expression and the production of IL-10 and reactive oxygen species (ROS).
At the same time, cincalcet also decreased the PMN apoptosis rate analyzed by flow cytometry.
However, CaSR inhibitor NPS-2143 and NF-κB signaling pathway inhibitor PDTC reverse the results cited earlier.
All of these results indicated that CaSR can regulate PMN functions and status to play a role in inflammation, which is probably through the NF-κB signaling pathway.
American Psychological Association (APA)
Zhai, Tai-yu& Cui, Bao-hong& Zou, Lei& Zeng, Jing-ya& Gao, Song& Zhao, Qianyu…[et al.]. 2017. Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1194471
Modern Language Association (MLA)
Zhai, Tai-yu…[et al.]. Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1194471
American Medical Association (AMA)
Zhai, Tai-yu& Cui, Bao-hong& Zou, Lei& Zeng, Jing-ya& Gao, Song& Zhao, Qianyu…[et al.]. Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1194471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194471