Autophagy Is a Protective Response to the Oxidative Damage to Endplate Chondrocytes in Intervertebral Disc: Implications for the Treatment of Degenerative Lumbar Disc
Joint Authors
Chen, Ke
Lv, Xiaohua
Li, Wei
Yu, Fei
Lin, Jianjing
Ma, Junxuan
Xiao, Deming
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Low back pain (LBP) is the leading cause of disability in the elderly.
Intervertebral disc degeneration (IDD) was considered as the main cause for LBP.
Degeneration of cartilaginous endplate was a crucial harmful factor during the initiation and development of IDD.
Oxidative stress was implicated in IDD.
However, the underlying molecular mechanism for the degeneration of cartilaginous endplate remains elusive.
Herein, we found that oxidative stress could induce apoptosis and autophagy in endplate chondrocytes evidenced by western blot analysis, flow cytometry, immunofluorescence staining, GFP-LC3B transfection, and MDC staining.
In addition, we also found that the apoptosis of endplate chondrocytes was significantly increased after the inhibition of autophagy by bafilomycin A1 shown by flow cytometry.
Furthermore, mTOR pathway upstream autophagy was greatly suppressed suggested by western blot assay.
In conclusion, our study strongly revealed that oxidative stress could increase autophagy and apoptosis of endplate chondrocytes in intervertebral disc.
The increase of autophagy activity could prevent endplate chondrocytes from apoptosis.
The autophagy in endplate chondrocytes induced by oxidative stress was mTOR dependent.
These findings might shed some new lights on the mechanism for IDD and provide new strategies for the treatments of IDD.
American Psychological Association (APA)
Chen, Ke& Lv, Xiaohua& Li, Wei& Yu, Fei& Lin, Jianjing& Ma, Junxuan…[et al.]. 2017. Autophagy Is a Protective Response to the Oxidative Damage to Endplate Chondrocytes in Intervertebral Disc: Implications for the Treatment of Degenerative Lumbar Disc. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194527
Modern Language Association (MLA)
Chen, Ke…[et al.]. Autophagy Is a Protective Response to the Oxidative Damage to Endplate Chondrocytes in Intervertebral Disc: Implications for the Treatment of Degenerative Lumbar Disc. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1194527
American Medical Association (AMA)
Chen, Ke& Lv, Xiaohua& Li, Wei& Yu, Fei& Lin, Jianjing& Ma, Junxuan…[et al.]. Autophagy Is a Protective Response to the Oxidative Damage to Endplate Chondrocytes in Intervertebral Disc: Implications for the Treatment of Degenerative Lumbar Disc. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194527
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194527