Reactive Oxygen Species Mediated Prostaglandin E2 Contributes to Acute Response of Epithelial Injury
Joint Authors
Fu, Xiu-jun
Peng, Ying-Bo
Hu, Yi-Ping
Yao, Min
Zhang, Yi-Fan
Wang, Ying
Yu, Wei-Rong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Reactive oxygen species (ROS) generated after tissue injury play a crucial role during wound healing through initiating acute inflammation, clarifying infection and dead tissue, and mediating various intracellular signal transduction.
Prostaglandin E2 (PGE2) has been identified as one of the major factors responsible for inflammation and tissue repair.
In this study, we tested our hypothesis that ROS produced by damaged human keratinocytes induces the synthesis of PGE2.
In vitro epithelial wounding model was used to observe the production of ROS and secretion of PGE2 as well as the involved signal pathway.
The mechanical injury caused the rapid production of ROS in in vitro cultured keratinocytes, which was significantly blocked by an inhibitor of nicotinamide adenine dinucleotide phosphate oxidase.
The increased intracellular ROS caused by mechanical injury stimulates PGE2 production in a time-dependent manner via the activation of cyclooxygenase-2 (COX-2), which was stimulated by phosphorylation of extracellular signal-regulated protein kinase (ERK).
These results indicate ROS-induced ERK activation leading to the activation of COX-2 and the synthesis of PGE2 in human keratinocytes responding to mechanical injury in the acute phase.
American Psychological Association (APA)
Hu, Yi-Ping& Peng, Ying-Bo& Zhang, Yi-Fan& Wang, Ying& Yu, Wei-Rong& Yao, Min…[et al.]. 2017. Reactive Oxygen Species Mediated Prostaglandin E2 Contributes to Acute Response of Epithelial Injury. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1194571
Modern Language Association (MLA)
Hu, Yi-Ping…[et al.]. Reactive Oxygen Species Mediated Prostaglandin E2 Contributes to Acute Response of Epithelial Injury. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1194571
American Medical Association (AMA)
Hu, Yi-Ping& Peng, Ying-Bo& Zhang, Yi-Fan& Wang, Ying& Yu, Wei-Rong& Yao, Min…[et al.]. Reactive Oxygen Species Mediated Prostaglandin E2 Contributes to Acute Response of Epithelial Injury. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1194571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194571