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Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
Joint Authors
Aguilar-Diosdado, Manuel
Segundo, Carmen
Mateos, Rosa María
Visiedo, Francisco
Santos-Rosendo, Celeste
Gil-Sánchez, M. del Mar
Bugatto, Fernando
López-Tinoco, Cristina
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM).
The role of NO and its placental targets in GDM pregnancies has yet to be determined.
S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO).
Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n=8) and GDM (n=8) pregnancies.
S-Nitrosylation was measured using the biotin-switch assay, while the expression and protein activity were assessed by immunoblotting and colorimetric methods, respectively.
Results indicated that catalase and peroxiredoxin nitrosylation levels were greater in GDM placentas, and that was accompanied by reduced catalase activity.
S-Nitrosylation of ERK1/2 and AKT was increased in GDM placentas, and their activities were inhibited.
Activities of caspase-3 and caspase-9 were increased, with the latter also showing diminished nitrosylation levels.
These findings suggest that S-nitrosylation is a little-known, but critical, mechanism by which NO directly modulates key placental proteins in women with GDM and, as a consequence, maternal and fetal anomalies during pregnancy can occur.
American Psychological Association (APA)
Visiedo, Francisco& Santos-Rosendo, Celeste& Mateos, Rosa María& Gil-Sánchez, M. del Mar& Bugatto, Fernando& Aguilar-Diosdado, Manuel…[et al.]. 2017. Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1194996
Modern Language Association (MLA)
Visiedo, Francisco…[et al.]. Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1194996
American Medical Association (AMA)
Visiedo, Francisco& Santos-Rosendo, Celeste& Mateos, Rosa María& Gil-Sánchez, M. del Mar& Bugatto, Fernando& Aguilar-Diosdado, Manuel…[et al.]. Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1194996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194996