Protective Effects of Methane-Rich Saline on Rats with Lipopolysaccharide-Induced Acute Lung Injury
Joint Authors
Ye, Xiaojian
Wang, WeiHeng
Sun, Aijun
Wang, Yang
Yang, Xiangqun
Ye, Zhouheng
Zhang, Chuansen
Sun, Xuejun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
The aim of this research is to evaluate the protective effects of methane-rich saline (MS) on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) and investigate its potential antioxidative, anti-inflammatory, and antiapoptotic activities.
Methods.
LPS-induced (20 mg/kg) ALI rats were injected with MS (2 ml/kg and 20 ml/kg) before the initiation of LPS induction.
Survival rate was determined until 96 h after LPS was induced.
Lung injury was assayed by oxygenation index, lung permeability index (LPI), wet-to-dry weight (W/D), and histology.
The cells in the bronchoalveolar lavage fluid (BALF) were counted.
Oxidative stress was examined by the level of malondialdehyde (MDA) and superoxide dismutase (SOD).
Inflammatory factors including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in BALF were determined by ELISA.
Lung tissue apoptosis was detected by TUNEL staining and western blotting of caspase-3.
Results.
It was found that methane significantly prolonged the rat survival, decreased the lung W/D ratio and the content of the inflammatory factors, and reduced the amount of caspase-3 and apoptotic index.
In addition, MS increased the level of SOD and decreased the level of MDA significantly.
Conclusions.
MS protects the LPS-challenged ALI via antioxidative, anti-inflammatory, and antiapoptotic effect, which may prove to be a novel therapy for the clinical management of ALI.
American Psychological Association (APA)
Sun, Aijun& Wang, WeiHeng& Ye, Xiaojian& Wang, Yang& Yang, Xiangqun& Ye, Zhouheng…[et al.]. 2017. Protective Effects of Methane-Rich Saline on Rats with Lipopolysaccharide-Induced Acute Lung Injury. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195656
Modern Language Association (MLA)
Sun, Aijun…[et al.]. Protective Effects of Methane-Rich Saline on Rats with Lipopolysaccharide-Induced Acute Lung Injury. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1195656
American Medical Association (AMA)
Sun, Aijun& Wang, WeiHeng& Ye, Xiaojian& Wang, Yang& Yang, Xiangqun& Ye, Zhouheng…[et al.]. Protective Effects of Methane-Rich Saline on Rats with Lipopolysaccharide-Induced Acute Lung Injury. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195656
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195656