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Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma
Joint Authors
Goloni-Bertollo, Eny Maria
Pavarino, Érika Cristina
Russo, Anelise
Biselli-Chicote, Patrícia M.
Kawasaki-Oyama, Rosa S.
Castanhole-Nunes, Márcia M. U.
Maniglia, José V.
Santi Neto, Dalísio de
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Introduction.
Differential expression of genes encoding cytochrome P450 (CYP) and other oxygenases enzymes involved in biotransformation mechanisms of endogenous and exogenous compounds can lead to oral tumor development.
Objective.
We aimed to identify the expression profile of these genes, searching for susceptibility biomarkers in oral squamous cell carcinoma.
Patients and Methods.
Sixteen oral squamous cell carcinoma samples were included in this study (eight tumor and eight adjacent non-tumor tissues).
Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR.
Protein quantification was performed by ELISA and IHC methods.
Bioinformatics tools were used to find metabolic pathways related to the enzymes encoded by differentially expressed genes.
Results.
CYP27B1, CYP27A1, CYP2E1, CYP2R1, CYP2J2, CYP2U1, CYP4F12, CYP4X1, CYP4B1, PTGIS, ALOX12, and MAOB genes presented differential expression in the oral tumors.
After correction by multiple tests, only the PTGIS (Prostaglandin I2 Synthase) gene presented significant differential expression (P < 0.05).
The PTGIS gene and protein were reduced in oral tumors.
Conclusion.
PTGIS presents downexpression in oral tumors.
PTGIS play an important role in the arachidonic acid metabolism.
Arachidonic acid and/or metabolites are derived from this pathway, which can influence the regulation of important physiological mechanisms in tumorigenesis process.
American Psychological Association (APA)
Russo, Anelise& Biselli-Chicote, Patrícia M.& Kawasaki-Oyama, Rosa S.& Castanhole-Nunes, Márcia M. U.& Maniglia, José V.& Santi Neto, Dalísio de…[et al.]. 2018. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma. Journal of Oncology،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1195830
Modern Language Association (MLA)
Russo, Anelise…[et al.]. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma. Journal of Oncology No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1195830
American Medical Association (AMA)
Russo, Anelise& Biselli-Chicote, Patrícia M.& Kawasaki-Oyama, Rosa S.& Castanhole-Nunes, Márcia M. U.& Maniglia, José V.& Santi Neto, Dalísio de…[et al.]. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma. Journal of Oncology. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1195830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195830