Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity

Joint Authors

Zhao, Wei
Xu, Shi-Yuan
Li, Le
Li, Fengxian
Li, Yu-jie
Yu, Xu-jiao
Liu, Zi-ting

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-06-08

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Biology

Abstract EN

Bupivacaine has been shown to induce neurotoxicity through inducing excessive reactive oxygen species (ROS), but the underlying mechanism remains unclear.

NOX2 is one of the most important sources of ROS in the nervous system, and its activation requires the membrane translocation of subunit p47phox.

However, the role of p47phox in bupivacaine-induced neurotoxicity has not been explored.

In our in vitro study, cultured human SH-SY5Y neuroblastoma cells were treated with 1.5 mM bupivacaine to induce neurotoxicity.

Membrane translocation of p47phox was assessed by measuring the cytosol/membrane ratio of p47phox.

The effects of the NOX inhibitor VAS2870 and p47phox-siRNA on bupivacaine-induced neurotoxicity were investigated.

Furthermore, the effect of VAS2870 on bupivacaine-induced neurotoxicity was assessed in vivo in rats.

All these changes were reversed by pretreatment with VAS2870 or transfection with p47phox-siRNA in SH-SY5Y cells.

Similarly, pretreatment with VAS2870 attenuated bupivacaine-induced neuronal toxicity in rats.

It is concluded that enhancing p47phox membrane translocation is a major mechanism whereby bupivacaine induced neurotoxicity and that pretreatment with VAS2870 or local p47phox gene knockdown attenuated bupivacaine-induced neuronal cell injury.

American Psychological Association (APA)

Li, Yu-jie& Zhao, Wei& Yu, Xu-jiao& Li, Fengxian& Liu, Zi-ting& Li, Le…[et al.]. 2017. Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1196210

Modern Language Association (MLA)

Li, Yu-jie…[et al.]. Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1196210

American Medical Association (AMA)

Li, Yu-jie& Zhao, Wei& Yu, Xu-jiao& Li, Fengxian& Liu, Zi-ting& Li, Le…[et al.]. Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1196210

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1196210