Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response
Joint Authors
Ermakov, Aleksei V.
Izevskaya, Vera L.
Ershova, Elizaveta
Veiko, Natalia
Kutsev, S. I.
Kaliyanov, Andrew A.
Sergeeva, Vasilina A.
Malinovskaya, E. M.
Kameneva, L. V.
Stukalov, S. V.
Dolgikh, O. A.
Veiko, V. P.
Konkova, Marina S.
Kostyuk, Svetlana V.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-22
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
We have hypothesized that the adaptive response to low doses of ionizing radiation (IR) is mediated by oxidized cell-free DNA (cfDNA) fragments.
Here, we summarize our experimental evidence for this model.
Studies involving measurements of ROS, expression of the NOX (superoxide radical production), induction of apoptosis and DNA double-strand breaks, antiapoptotic gene expression and cell cycle inhibition confirm this hypothesis.
We have demonstrated that treatment of mesenchymal stem cells (MSCs) with low doses of IR (10 cGy) leads to cell death of part of cell population and release of oxidized cfDNA.
cfDNA has the ability to penetrate into the cytoplasm of other cells.
Oxidized cfDNA, like low doses of IR, induces oxidative stress, ROS production, ROS-induced oxidative modifications of nuclear DNA, DNA breaks, arrest of the cell cycle, activation of DNA reparation and antioxidant response, and inhibition of apoptosis.
The MSCs pretreated with low dose of irradiation or oxidized cfDNA were equally effective in induction of adaptive response to challenge further dose of radiation.
Our studies suggest that oxidized cfDNA is a signaling molecule in the stress signaling that mediates radiation-induced bystander effects and that it is an important component of the development of radioadaptive responses to low doses of IR.
American Psychological Association (APA)
Sergeeva, Vasilina A.& Ershova, Elizaveta& Veiko, Natalia& Malinovskaya, E. M.& Kaliyanov, Andrew A.& Kameneva, L. V.…[et al.]. 2017. Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-22.
https://search.emarefa.net/detail/BIM-1196580
Modern Language Association (MLA)
Sergeeva, Vasilina A.…[et al.]. Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-22.
https://search.emarefa.net/detail/BIM-1196580
American Medical Association (AMA)
Sergeeva, Vasilina A.& Ershova, Elizaveta& Veiko, Natalia& Malinovskaya, E. M.& Kaliyanov, Andrew A.& Kameneva, L. V.…[et al.]. Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-22.
https://search.emarefa.net/detail/BIM-1196580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196580