![](/images/graphics-bg.png)
Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes
Joint Authors
Galland, Fabiana
Leite, Marina Concli
Hansen, Fernanda
Lirio, Franciane
de Souza, Daniela Fraga
Da Ré, Carollina
Pacheco, Rafaela Ferreira
Vizuete, Adriana Fernanda
Quincozes-Santos, André
Gonçalves, Carlos Alberto
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The impairment of astrocyte functions is associated with diabetes mellitus and other neurodegenerative diseases.
Astrocytes have been proposed to be essential cells for neuroprotection against elevated levels of methylglyoxal (MG), a highly reactive aldehyde derived from the glycolytic pathway.
MG exposure impairs primary astrocyte viability, as evaluated by different assays, and these cells respond to MG elevation by increasing glyoxalase 1 activity and glutathione levels, which improve cell viability and survival.
However, C6 glioma cells have shown strong signs of resistance against MG, without significant changes in the glyoxalase system.
Results for aminoguanidine coincubation support the idea that MG toxicity is mediated by glycation.
We found a significant decrease in glutamate uptake by astrocytes, without changes in the expression of the major transporters.
Carbenoxolone, a nonspecific inhibitor of gap junctions, prevented the cytotoxicity induced by MG in astrocyte cultures.
Thus, our data reinforce the idea that astrocyte viability depends on gap junctions and that the impairment induced by MG involves glutamate excitotoxicity.
The astrocyte susceptibility to MG emphasizes the importance of this compound in neurodegenerative diseases, where the neuronal damage induced by MG may be aggravated by the commitment of the cells charged with MG clearance.
American Psychological Association (APA)
Hansen, Fernanda& Galland, Fabiana& Lirio, Franciane& de Souza, Daniela Fraga& Da Ré, Carollina& Pacheco, Rafaela Ferreira…[et al.]. 2017. Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1196600
Modern Language Association (MLA)
Hansen, Fernanda…[et al.]. Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1196600
American Medical Association (AMA)
Hansen, Fernanda& Galland, Fabiana& Lirio, Franciane& de Souza, Daniela Fraga& Da Ré, Carollina& Pacheco, Rafaela Ferreira…[et al.]. Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1196600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196600