Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders

Joint Authors

Wysocka, Ewa
Michalak, Slawomir
Rybacka-Mossakowska, Joanna
Ambrosius, Wojciech
Kozubski, Wojciech
Florczak-Wyspiańska, Jolanta
Osztynowicz, Krystyna
Baszczuk, Aleksandra

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-09-10

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Objective.

Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders.

Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties.

The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the SIRT3 activity in patients with movement disorders.

Methods.

Mitochondrial respiration was analyzed in intact PBMCs using the ROUTINE, LEAK, electron transfer system (ETS), and residual oxygen consumption (ROX) protocol by means of high-resolution respirometry.

The SIRT3 expression and PBMC activity were measured using fluorometry.

Ultrasound measurements of the echogenicity of the substantia nigra and the diameter of the 3rd ventricle were also performed.

Results.

Patients with movement disorders exhibited a lower ROUTINE respiration than controls (P = 0.0237).

Reduced oxygen fluxes in the LEAK (P=0.033) and ROX (P=0.0486) states were observed in patients with movement disorders compared with controls.

Decreased ROUTINE respiration (P=0.007) and oxygen flux in the LEAK state (P=0.0203) were observed in patients with PD with substantia nigra hyperechogenicity compared with controls.

Decreased SIRT 3 deacetylase activity was found in patients with movement disorders.

Conclusion.

Impaired mitochondrial respiration in intact PBMCs was associated with inhibited SIRT3 activity and neurodegeneration measures evaluated using ultrasound in patients with PD.

American Psychological Association (APA)

Michalak, Slawomir& Florczak-Wyspiańska, Jolanta& Rybacka-Mossakowska, Joanna& Ambrosius, Wojciech& Osztynowicz, Krystyna& Baszczuk, Aleksandra…[et al.]. 2017. Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196658

Modern Language Association (MLA)

Michalak, Slawomir…[et al.]. Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1196658

American Medical Association (AMA)

Michalak, Slawomir& Florczak-Wyspiańska, Jolanta& Rybacka-Mossakowska, Joanna& Ambrosius, Wojciech& Osztynowicz, Krystyna& Baszczuk, Aleksandra…[et al.]. Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196658

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1196658