Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1Smad2-Mediated Endothelial-to-Mesenchymal Transition
Joint Authors
Tang, Qi-Zhu
Zhang, Ning
Wu, Qing-Qing
Xiao, Yang
Jin, Ya-Ge
Yuan, Yuan
Fan, Di
Che, Yan
Wang, Zhao-Peng
Wang, Sha-Sha
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Puerarin is a kind of flavonoids and is extracted from Chinese herb Kudzu root.
Puerarin is widely used as an adjuvant therapy in Chinese clinics.
But little is known about its effects on regulating cardiac fibrosis.
Methods.
Mice were subjected to transverse aorta constriction (TAC) for 8 weeks; meanwhile puerarin was given 1 week after TAC.
Cardiac fibrosis was assessed by pathological staining.
The mRNA and protein changes of CD31 and vimentin in both animal and human umbilical vein endothelial cells (HUVECs) models were detected.
Immunofluorescence colocalization of CD31 and vimentin and scratch test were carried out to examine TGF-β1-induced changes in HUVECs.
The agonist and antagonist of peroxisome proliferator-activated receptor-γ (PPAR-γ) were used to explore the underlying mechanism.
Results.
Puerarin mitigated TAC-induced cardiac fibrosis, accompanied with suppressed endothelial-to-mesenchymal transition (EndMT).
The consistent results were achieved in HUVECs model.
TGF-β1/Smad2 signaling pathway was blunted and PPAR-γ expression was upregulated in puerarin-treated mice and HUVECs.
Pioglitazone could reproduce the protective effect in HUVECs, while GW9662 reversed this effect imposed by puerarin.
Conclusion.
Puerarin protected against TAC-induced cardiac fibrosis, and this protective effect may be attributed to the upregulation of PPAR-γ and the inhibition of TGF-β1/Smad2-mediated EndMT.
American Psychological Association (APA)
Jin, Ya-Ge& Yuan, Yuan& Wu, Qing-Qing& Zhang, Ning& Fan, Di& Che, Yan…[et al.]. 2017. Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1Smad2-Mediated Endothelial-to-Mesenchymal Transition. PPAR Research،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1197203
Modern Language Association (MLA)
Jin, Ya-Ge…[et al.]. Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1Smad2-Mediated Endothelial-to-Mesenchymal Transition. PPAR Research No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1197203
American Medical Association (AMA)
Jin, Ya-Ge& Yuan, Yuan& Wu, Qing-Qing& Zhang, Ning& Fan, Di& Che, Yan…[et al.]. Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1Smad2-Mediated Endothelial-to-Mesenchymal Transition. PPAR Research. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1197203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197203