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Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability
Joint Authors
Tan, Jun Jie
Devasvaran, Kogilavanee
Ng, Chin Theng
Fong, Lai Yen
Yong, Yoke Keong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties.
However, the possible vascular protective effect of TH against oxidative stress remains unclear.
In this study, the effects of TH on hydrogen peroxide- (H2O2-) elicited vascular hyperpermeability in human umbilical vein endothelial cells (HUVECs) and Balb/c mice were evaluated.
Our data showed that TH concentrations ranging from 0.01% to 1.00% showed no cytotoxic effect to HUVECs.
Induction with 0.5 mM H2O2 was found to increase HUVEC permeability, but the effect was significantly reversed attenuated by TH (p<0.05), of which the permeability with the highest inhibition peaked at 0.1%.
In Balb/c mice, TH (0.5 g/kg-1.5 g/kg) significantly (p<0.05) reduced H2O2 (0.3%)-induced albumin-bound Evans blue leak, in a dose-dependent manner.
Immunofluorescence staining confirmed that TH reduced actin stress fiber formation while increasing cortical actin formation and colocalization of caveolin-1 and β-catenin in HUVECs.
Signaling studies showed that HUVECs pretreated with TH significantly (p<0.05) decreased intracellular calcium release, while sustaining the level of cAMP when challenged with H2O2.
These results suggested that TH could inhibit H2O2-induced vascular hyperpermeability in vitro and in vivo by suppression of adherence junction protein redistribution via calcium and cAMP, which could have a therapeutic potential for diseases related to the increase of both oxidant and vascular permeability.
American Psychological Association (APA)
Devasvaran, Kogilavanee& Tan, Jun Jie& Ng, Chin Theng& Fong, Lai Yen& Yong, Yoke Keong. 2019. Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202032
Modern Language Association (MLA)
Devasvaran, Kogilavanee…[et al.]. Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1202032
American Medical Association (AMA)
Devasvaran, Kogilavanee& Tan, Jun Jie& Ng, Chin Theng& Fong, Lai Yen& Yong, Yoke Keong. Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202032
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202032