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The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow
Joint Authors
Kheolamai, Pakpoom
Manochantr, Sirikul
Tantrawatpan, Chairat
Tantikanlayaporn, Duangrat
Marupanthorn, Kulisara
Sanguanjit, Prakasit
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-19
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Mesenchymal stromal cells (MSCs) offering valuable anticipations for the treatment of degenerative diseases.
They can be found in many tissues including amnion.
MSCs from amnion (AM-MSCs) can differentiate into osteoblast similar to that of bone marrow-derived MSCs (BM-MSCs).
However, the ability is not much efficient compared to BM-MSCs.
This study aimed to examine the effects of BMP-2 and miRNAs on osteogenic differentiation of AM-MSCs compared to those of BM-MSCs.
The osteogenic differentiation capacity after miRNA treatment was assessed by ALP expression, ALP activity, and osteogenic marker gene expression.
The results showed that the osteogenic differentiation capacity increased after BMP-2 treatment both in AM-MSCs and BM-MSCs.
MiR-31, miR-106a, and miR-148a were downregulated during the osteogenic differentiation.
After transfection with anti-miRNAs, ALP activity and osteogenic genes were increased over the time of differentiation.
The data lead to the potential for using AM-MSCs as an alternative source for bone regeneration.
Moreover, the information of miRNA expression and function during osteogenic differentiation may be useful for the development of new therapeutics or enhanced an in vitro culture technique required for stem cell-based therapies in the bone regeneration.
American Psychological Association (APA)
Manochantr, Sirikul& Marupanthorn, Kulisara& Tantrawatpan, Chairat& Kheolamai, Pakpoom& Tantikanlayaporn, Duangrat& Sanguanjit, Prakasit. 2017. The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202051
Modern Language Association (MLA)
Manochantr, Sirikul…[et al.]. The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1202051
American Medical Association (AMA)
Manochantr, Sirikul& Marupanthorn, Kulisara& Tantrawatpan, Chairat& Kheolamai, Pakpoom& Tantikanlayaporn, Duangrat& Sanguanjit, Prakasit. The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202051
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202051