Bailcalin Protects against Diabetic Cardiomyopathy through Keap1Nrf2AMPK-Mediated Antioxidative and Lipid-Lowering Effects
Joint Authors
Wang, Fang
Shan, Yingguang
Li, Ran
Liu, Yuan
Gao, Lu
Qiu, Chun-guang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-01
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Previous studies demonstrated that Bailcalin (BAI) prevented cardiac injuries under different disease models.
Whether BAI protected against type 2 diabetes mellitus- (T2DM-) associated cardiomyopathy was investigated in this study.
T2DM was established by the combination of streptozotocin injection and high-fat diet in mice.
BAI was administered daily for 6 months.
After evaluating cardiac functions, mice hearts were removed and processed for morphological, biochemical, and molecular mechanism analyses.
Neonatal rat cardiomyocytes (NRCM) were isolated and treated with high glucose and palmitate (HG/Pal) for in vitro investigation.
BAI significantly ameliorated T2DM-induced cardiomyocyte hypertrophy, interstitial fibrosis, and lipid accumulation accompanied by markedly improved cardiac functions in diabetic mice.
Mechanically, BAI restored decreased phosphorylation of AMPK and enhanced expression and nuclei translocation of Nrf2.
In in vitro experiments, BAI also prevented NRCM from HG/Pal-induced apoptosis and oxidative stress injuries by increasing p-AMPK and Nrf2 accumulation.
The means by which BAI restored p-AMPK seemed to be related to the antioxidative effects of Nrf2 after silencing AMPK or Nrf2 in NRCM.
Furthermore, BAI regulated Nrf2 by inhibiting Nrf2 ubiquitination and consequent degradation mediated by Keap1.
This study showed that BAI alleviated diabetes-associated cardiac dysfunction and cardiomyocyte injuries in vivo and in vitro via Keap1/Nrf2/AMPK-mediated antioxidation and lipid-lowering effects.
BAI might be a potential adjuvant drug for diabetes cardiomyopathy treatment.
American Psychological Association (APA)
Li, Ran& Liu, Yuan& Shan, Yingguang& Gao, Lu& Wang, Fang& Qiu, Chun-guang. 2019. Bailcalin Protects against Diabetic Cardiomyopathy through Keap1Nrf2AMPK-Mediated Antioxidative and Lipid-Lowering Effects. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1203154
Modern Language Association (MLA)
Li, Ran…[et al.]. Bailcalin Protects against Diabetic Cardiomyopathy through Keap1Nrf2AMPK-Mediated Antioxidative and Lipid-Lowering Effects. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1203154
American Medical Association (AMA)
Li, Ran& Liu, Yuan& Shan, Yingguang& Gao, Lu& Wang, Fang& Qiu, Chun-guang. Bailcalin Protects against Diabetic Cardiomyopathy through Keap1Nrf2AMPK-Mediated Antioxidative and Lipid-Lowering Effects. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1203154
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203154