Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway

Joint Authors

Lin, Zhen-Lang
Cai, Chen-chen
Zhu, Jiang-hu
Ye, Li-xia
Dai, Yuan-yuan
Fang, Ming-chu
Hu, Ying-ying
Pan, Shu-lin
Chen, Si
Li, Pei-jun
Fu, Xiao-qin

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-29, 29 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-02-06

Country of Publication

Egypt

No. of Pages

29

Main Subjects

Biology

Abstract EN

Hypoxic-ischemic encephalopathy (HIE) is detrimental to newborns and is associated with high mortality and poor prognosis.

Thus, the primary aim of the present study was to determine whether glycine could (1) attenuate HIE injury in rats and hypoxic stress in PC12 cells and (2) downregulate mitochondria-mediated autophagy dependent on the adenosine monophosphate- (AMP-) activated protein kinase (AMPK) pathway.

Experiments conducted using an in vivo HIE animal model and in vitro hypoxic stress to PC12 cells revealed that intense autophagy associated with mitochondrial function occurred during in vivo HIE injury and in vitro hypoxic stress.

However, glycine treatment effectively attenuated mitochondria-mediated autophagy.

Additionally, after identifying alterations in proteins within the AMPK pathway in rats and PC12 cells following glycine treatment, cyclosporin A (CsA) and 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside (AICAR) were administered in these models and indicated that glycine protected against HIE and CoCl2 injury by downregulating mitochondria-mediated autophagy that was dependent on the AMPK pathway.

Overall, glycine attenuated hypoxic-ischemic injury in neurons via reductions in mitochondria-mediated autophagy through the AMPK pathway both in vitro and in vivo.

American Psychological Association (APA)

Cai, Chen-chen& Zhu, Jiang-hu& Ye, Li-xia& Dai, Yuan-yuan& Fang, Ming-chu& Hu, Ying-ying…[et al.]. 2019. Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-29.
https://search.emarefa.net/detail/BIM-1203532

Modern Language Association (MLA)

Cai, Chen-chen…[et al.]. Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-29.
https://search.emarefa.net/detail/BIM-1203532

American Medical Association (AMA)

Cai, Chen-chen& Zhu, Jiang-hu& Ye, Li-xia& Dai, Yuan-yuan& Fang, Ming-chu& Hu, Ying-ying…[et al.]. Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-29.
https://search.emarefa.net/detail/BIM-1203532

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1203532