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Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs
Joint Authors
Yu, Jie
Gu, Wen
Yang, Xingxin
Liang, Li
Li, Feng-Jiao
Liu, Xin
Dong, Jin-Cai
Zeng, Lin-Xi
Mu, Jian-Kang
Bi, Qian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-09
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Targeting mitochondria as a hepatic-protective strategy has gained attention, because of their important roles in energy production, adjustment of apoptosis, and generation of reactive oxygen species.
To promote the discovery of natural mitochondria-targeted hepatic-protectants, we established a hepatocellular mitochondria-based capturing method by coupling affinity ultrafiltration with liquid chromatography/mass spectrometry (LC/MS), which is suitable for identifying mitochondrial ligands from medicinal herbs (MHs).
After evaluating the feasibility of the method, it was applied for capturing mitochondria-targeting constituents from Peucedani Radix extract.
A total of 10 active compounds were identified by LC/MS, all of which were newly identified mitochondrial ligands.
The mitochondria-remedying activity of 4 of the 10 hits was confirmed by pharmacological tests in vitro.
Additionally, the hepatic-protective abilities of 4 hits were verified in both carbon tetrachloride-damaged liver L02 cells and mice.
These results indicated that the method could be used for identifying hepatic mitochondria-targeting constituents in MHs, which might be beneficial for hepatic-protective development.
American Psychological Association (APA)
Yang, Xingxin& Liang, Li& Liu, Xin& Li, Feng-Jiao& Dong, Jin-Cai& Mu, Jian-Kang…[et al.]. 2019. Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1203565
Modern Language Association (MLA)
Yang, Xingxin…[et al.]. Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1203565
American Medical Association (AMA)
Yang, Xingxin& Liang, Li& Liu, Xin& Li, Feng-Jiao& Dong, Jin-Cai& Mu, Jian-Kang…[et al.]. Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1203565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203565