Stimulation of Na+K+-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPKSIRT3PPARγ Signaling Pathway
Joint Authors
Bian, Jin-Song
Xiong, Siping
Sun, Hai-Jian
Cao, Lei
Zhu, Mengyuan
Liu, Tengteng
Wu, Zhiyuan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-15
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Activation of the renin-angiotensin system (RAS) contributes to the pathogenesis of cardiovascular diseases.
Sodium potassium ATPase (NKA) expression and activity are often regulated by angiotensin II (Ang II).
This study is aimed at investigating whether DR-Ab, an antibody against 4th extracellular region of NKA, can protect Ang II-induced cardiomyocyte hypertrophy.
Our results showed that Ang II treatment significantly reduced NKA activity and membrane expression.
Pretreatment with DR-Ab preserved cell size in Ang II-induced cardiomyopathy by stabilizing the plasma membrane expression of NKA and restoring its activity.
DR-Ab reduced intracellular ROS generation through inhibition of NADPH oxidase activity and protection of mitochondrial functions in Ang II-treated H9c2 cardiomyocytes.
Pharmacological manipulation and Western blotting analysis demonstrated the cardioprotective effects were mediated by the activation of the AMPK/Sirt-3/PPARγ signaling pathway.
Taken together, our results suggest that dysfunction of NKA is an important mechanism for Ang II-induced cardiomyopathy and DR-Ab may be a novel and promising therapeutic approach to treat cardiomyocyte hypertrophy.
American Psychological Association (APA)
Xiong, Siping& Sun, Hai-Jian& Cao, Lei& Zhu, Mengyuan& Liu, Tengteng& Wu, Zhiyuan…[et al.]. 2019. Stimulation of Na+K+-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPKSIRT3PPARγ Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1203745
Modern Language Association (MLA)
Xiong, Siping…[et al.]. Stimulation of Na+K+-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPKSIRT3PPARγ Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1203745
American Medical Association (AMA)
Xiong, Siping& Sun, Hai-Jian& Cao, Lei& Zhu, Mengyuan& Liu, Tengteng& Wu, Zhiyuan…[et al.]. Stimulation of Na+K+-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPKSIRT3PPARγ Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1203745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203745