Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis
Joint Authors
Hu, Wenjing
Geng, Shan
Xu, Shangcheng
Li, Ke
Yang, Mengliu
Han, Hongdong
Zhou, Baoyong
Fan, Xiaoyun
Yang, Gangyi
Liu, Yongsheng
Liu, Hua
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background and Objectives.
Genome-wide association studies (GWAS) have shown that cartilage intermediate layer protein 2 (CILP2) is associated with blood lipid levels and coronary heart disease (CHD).
However, no study has reported whether CILP2 is related to atherosclerosis in humans.
The purpose of the current study is to identify the associations between CILP2 and atherosclerosis in vitro and in vivo.
Methods and Results.
Circulating CILP2 levels (measured by ELISA) were compared to various insulin resistance- and atherosclerosis-related parameters in normal subjects and newly diagnosed CHD patients.
THP-1 cells were cultured and treated with indicated stimulators.
Western blots and RT-PCR were performed to examine protein and mRNA expressions.
The results showed that there were significantly higher circulating CILP2 levels in CHD patients relative to healthy controls.
Circulating CILP2 correlated positively with waist-hip ratio (WHR), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), HbA1c, homeostasis model assessment of insulin resistance (HOMA-IR), and Gensini scores.
In an in vitro study, we found that CILP2 increased oxidatively modified LDL-stimulated lipid accumulation in THP-1 macrophages via the upregulation of CD36 expression.
Inhibition of PPARγ signaling eliminated the CILP2 regulation of CD36 expression in THP-1 macrophages.
CILP2 positively regulated CD36 transcription through PPARγ-mediated action on two peroxisome-proliferator-responsive elements (PPREs) binding sites of CD36 promoter, PPRE-G, and PPRE-J.
Conclusions.
Our findings have uncovered a novel role for CILP2 in lipid uptake and foam cell formation.
This role is mediated by CD36 through the activation of PPARγ pathway.
American Psychological Association (APA)
Hu, Wenjing& Li, Ke& Han, Hongdong& Geng, Shan& Zhou, Baoyong& Fan, Xiaoyun…[et al.]. 2020. Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1203844
Modern Language Association (MLA)
Hu, Wenjing…[et al.]. Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1203844
American Medical Association (AMA)
Hu, Wenjing& Li, Ke& Han, Hongdong& Geng, Shan& Zhou, Baoyong& Fan, Xiaoyun…[et al.]. Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1203844
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203844