Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome
Joint Authors
Lu, Hung-I
Sheu, Jiunn-Jye
Chen, Yi-Ling
Lin, Kun-Chen
Yin, Tsung-Cheng
Sung, Pei-Hsun
Chen, Kuan-Hung
Chai, Han-Tan
Chen, Chih-Hung
Li, Yi-Chen
Shao, Pei-Lin
Lee, Mel S.
Wallace, Christopher Glenn
Yip, Hon-Kan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-21
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
This study tested the hypothesis that shock wave therapy (SW) enhances mitochondrial uptake into the lung epithelial and parenchymal cells to attenuate lung injury from acute respiratory distress syndrome (ARDS).
ARDS was induced in rats through continuous inhalation of 100% oxygen for 48 h, while SW entailed application 0.15 mJ/mm2 for 200 impulses at 6 Hz per left/right lung field.
In vitro and ex vivo studies showed that SW enhances mitochondrial uptake into lung epithelial and parenchyma cells (all p<0.001).
Flow cytometry demonstrated that albumin levels and numbers of inflammatory cells (Ly6G+/CD14+/CD68+/CD11b/c+) in bronchoalveolar lavage fluid were the highest in untreated ARDS, were progressively reduced across SW, Mito, and SW + Mito (all p<0.0001), and were the lowest in sham controls.
The same profile was also seen for fibrosis/collagen deposition, levels of biomarkers of oxidative stress (NOX-1/NOX-2/oxidized protein), inflammation (MMP-9/TNF-α/NF-κB/IL-1β/ICAM-1), apoptosis (cleaved caspase 3/PARP), fibrosis (Smad3/TGF-β), mitochondrial damage (cytosolic cytochrome c) (all p<0.0001), and DNA damage (γ-H2AX+), and numbers of parenchymal inflammatory cells (CD11+/CD14+/CD40L+/F4/80+) (p<0.0001).
These results suggest that SW-assisted Mito therapy effectively protects the lung parenchyma from ARDS-induced injury.
American Psychological Association (APA)
Lin, Kun-Chen& Wallace, Christopher Glenn& Yin, Tsung-Cheng& Sung, Pei-Hsun& Chen, Kuan-Hung& Lu, Hung-I…[et al.]. 2018. Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1203881
Modern Language Association (MLA)
Lin, Kun-Chen…[et al.]. Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome. Mediators of Inflammation No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1203881
American Medical Association (AMA)
Lin, Kun-Chen& Wallace, Christopher Glenn& Yin, Tsung-Cheng& Sung, Pei-Hsun& Chen, Kuan-Hung& Lu, Hung-I…[et al.]. Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1203881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203881