Activation of Macrophages by Oligomeric Proteins of Different Size and Origin

Joint Authors

Zvirbliene, Aurelija
Dalgediene, Indre
Lučiūnaitė, Asta

Source

Mediators of Inflammation

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-11-18

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Diseases

Abstract EN

Activation of macrophages is one of the key processes in generating the immune response against pathogens or misfolded/aggregated otherwise unharmful host’s proteins.

Antigens and their immune complexes (IC) may shape macrophage phenotype in various directions.

Data on the impact of protein structure during inflammation are evident; however, some separate steps of this process involving changes in macrophage phenotype are not fully understood.

Our aim was to investigate the phenotype of macrophages after activation with different oligomeric proteins and their IC.

We have used amyloid beta (Aβ1–42) that plays a role in neurodegenerative inflammation as a model of host-associated protein and three oligomeric viral antigens as pathogen-associated proteins.

Murine cell lines J774, BV-2, and macrophage primary cell culture were treated with oligomeric proteins and their IC.

After 48 h, expression of surface markers F4/80, CD68, CD86, and CD206 and secreted cytokines IL-10, IL-12, IL-23, and TNF-α was analysed.

Aβ1–42 oligomers stimulated expression of both inflammatory and anti-inflammatory molecules; however, fibrils induced less intense expression of markers investigated as compared to small and large oligomers.

Two out of three viral oligomeric proteins induced the inflammatory response of macrophages.

Data suggest that macrophage activation pattern depends on the origin, size, and structure of oligomeric proteins.

American Psychological Association (APA)

Dalgediene, Indre& Lučiūnaitė, Asta& Zvirbliene, Aurelija. 2018. Activation of Macrophages by Oligomeric Proteins of Different Size and Origin. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1204187

Modern Language Association (MLA)

Dalgediene, Indre…[et al.]. Activation of Macrophages by Oligomeric Proteins of Different Size and Origin. Mediators of Inflammation No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1204187

American Medical Association (AMA)

Dalgediene, Indre& Lučiūnaitė, Asta& Zvirbliene, Aurelija. Activation of Macrophages by Oligomeric Proteins of Different Size and Origin. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1204187

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1204187