Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats
Joint Authors
Balgoon, Maha Jameal
Ayuob, Nasra
El-Mansy, Ahmed A.
Mubarak, Wafaa A.
Firgany, Alaa El-Din L.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-21
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
The association between hypothyroidism and renal diseases has been described in many studies.
Nigella Sativa was among the recently reported natural product that has the potential to prevent renal tissue damage and fibrosis.
The aim of this study was to evaluate the possible protective effect of thymoquinone on the structure of the renal cortex of hypothyroid rats and explore the mechanism behind it.
Methods.
An experimental model of hypothyroidism was induced in adult male Wistar rats by administration of propylthiouracil (6 mg/kg/body weight).
One hypothyroid group was treated with thymoquinone at the dose of 50 mg/kg/body weight and compared to the untreated group.
Thyroid function and oxidant/antioxidant status were assessed in the serum.
Catalase gene expression was assessed using the real-time polymerase chain reaction.
The kidney was assessed both histologically and immunohistochemically.
Results.
Administration of propylthiouracil resulted in a significant decrease in the serum levels of nitric oxide, reduced glutathione, and superoxide dismutase activity while the level of malondialdehyde significantly (p<0.001) increased.
Administration of thymoquinone alleviated this effect on the thyroid hormones and significantly increased the serum levels of antioxidants.
Thymoquinone significantly (p<0.001) upregulated catalase transcription by about 24-fold and could block the hypothyroidism-induced glomerular and tubular injury.
Conclusion.
Thymoquinone may have a potential protective effect against hypothyroidism-induced renal injury acting through the attenuation of the oxidative stress and upregulation of renal catalase gene expression.
American Psychological Association (APA)
Ayuob, Nasra& Balgoon, Maha Jameal& El-Mansy, Ahmed A.& Mubarak, Wafaa A.& Firgany, Alaa El-Din L.. 2020. Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1204237
Modern Language Association (MLA)
Ayuob, Nasra…[et al.]. Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1204237
American Medical Association (AMA)
Ayuob, Nasra& Balgoon, Maha Jameal& El-Mansy, Ahmed A.& Mubarak, Wafaa A.& Firgany, Alaa El-Din L.. Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1204237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204237