![](/images/graphics-bg.png)
Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress
Joint Authors
Valvassori, Samira S.
Dal-Pizzol, Felipe
Saba, Sumbal
Rafique, Jamal
Frizon, Tiago E. A.
Cararo, José H.
Dal-Pont, Gustavo C.
Michels, Monique
Braga, Hugo C.
Pimentel, Tairine
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Herein, we report the synthesis of novel selenocyanates and assessment of their effect on the oxidative challenge elicited by hydrogen peroxide (H2O2) in cultured mouse neurons.
First, α-methylene-β-hydroxy esters were prepared as precursors of allylic bromides.
A reaction involving the generated bromides and sodium selenocyanate was conducted to produce the desired selenocyanates (3a-f).
We next prepared cultures of neurons from 7-day-old mice (n=36).
H2O2 (10-5 M) was added into the culture flasks as an oxidative stress inducer, alone or combined with one of each designed compounds.
(PhSe)2 was used as a positive control.
It was carried out assessment of lipid (thiobarbituric acid reactive species, 4-hydroxy-2′-nonenal, 8-isoprostane), DNA (8-hydroxy-2′-deoxyguanosine), and protein (carbonyl) modification parameters.
Finally, catalase and superoxide dismutase activities were also evaluated.
Among the compounds, 3b, 3d, and 3f exhibited the most pronounced pattern of antioxidant activity, similar to (PhSe)2.
These novel aromatic selenocyanates could be promising to be tried in most sophisticated in vitro studies or even at the preclinical level.
American Psychological Association (APA)
Frizon, Tiago E. A.& Cararo, José H.& Saba, Sumbal& Dal-Pont, Gustavo C.& Michels, Monique& Braga, Hugo C.…[et al.]. 2020. Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1204855
Modern Language Association (MLA)
Frizon, Tiago E. A.…[et al.]. Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1204855
American Medical Association (AMA)
Frizon, Tiago E. A.& Cararo, José H.& Saba, Sumbal& Dal-Pont, Gustavo C.& Michels, Monique& Braga, Hugo C.…[et al.]. Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1204855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204855