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Icariin Inhibits AGE-Induced Injury in PC12 Cells by Directly Targeting Apoptosis Regulator Bax
Joint Authors
Zeng, Ke-Wu
Zhao, Shaoyang
Li, Weiwei
Liao, Li-Xi
Tu, Peng-Fei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetic encephalopathy (DE) is a serious complication caused by long-term cognitive impairment in diabetic patients.
At present, there is no effective treatment for DE.
Icariin (ICA) is a bioactive ingredient isolated from Epimedium.
Previous research indicated that ICA was neuroprotective against Aβ-induced PC12 cell insult; however, the effect of ICA on an advanced glycosylation end product- (AGE-) induced neural injury model has not been studied.
In this study, we investigated the neuroprotective effects of ICA on AGE-induced injury in PC12 cells.
Our findings revealed that ICA could effectively protect PC12 cells from AGE-induced cell apoptosis by suppressing oxidative stress.
Moreover, we observed that ICA could significantly protect against mitochondrial depolarization following AGE stimulation and inactivate the mitochondria-dependent caspase-9/3 apoptosis pathway.
Most notably, we identified the direct target protein of ICA as apoptosis regulator Bax by a pulldown assay.
We found that ICA could specifically target Bax protein and inhibit Bax dimer formation and migration to mitochondria.
Furthermore, a siRNA knockdown experiment revealed that ICA could inhibit PC12 cell apoptosis and oxidative stress through targeting Bax.
Taken together, our findings demonstrated that ICA could attenuate AGE-induced oxidative stress and mitochondrial apoptosis by specifically targeting Bax and further regulating the biological function of Bax on mitochondria.
American Psychological Association (APA)
Zhao, Shaoyang& Liao, Li-Xi& Tu, Peng-Fei& Li, Weiwei& Zeng, Ke-Wu. 2019. Icariin Inhibits AGE-Induced Injury in PC12 Cells by Directly Targeting Apoptosis Regulator Bax. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1205361
Modern Language Association (MLA)
Zhao, Shaoyang…[et al.]. Icariin Inhibits AGE-Induced Injury in PC12 Cells by Directly Targeting Apoptosis Regulator Bax. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1205361
American Medical Association (AMA)
Zhao, Shaoyang& Liao, Li-Xi& Tu, Peng-Fei& Li, Weiwei& Zeng, Ke-Wu. Icariin Inhibits AGE-Induced Injury in PC12 Cells by Directly Targeting Apoptosis Regulator Bax. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1205361
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205361