Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell
Joint Authors
Chen, Min
Chen, Min
Chen, Suren
Zhou, Jingjing
Dong, Fangfang
Shen, Zhiming
Wu, Haowei
Cui, Xiuhong
Gao, Fei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The interaction between germ cell and somatic cell plays important roles in germ cell development.
However, the exact function of gonad somatic cell in germ cell differentiation is unclear.
In the present study, the function of gonad somatic cell in germ cell meiosis was examined by using mouse models with aberrant somatic cell differentiation.
In Wt1R394W/R394W mice, the genital ridge is absent due to the apoptosis of coelomic epithelial cells.
Interestingly, in both male and female Wt1R394W/R394W germ cells, STRA8 was detected at E12.5 and the scattered SYCP3 foci were observed at E13.5 which was consistent with control females.
In Wt1-/flox; Cre-ERTM mice, Wt1 was inactivated by the injection of tamoxifen at E9.5 and the differentiation of Sertoli and granulosa cells was completely blocked.
We found that most germ cells were located outside of genital ridge after Wt1 inactivation.
STRA8, SYCP3, and γH2AX proteins were detected in germ cells of both male and female Wt1-/flox; Cre-ERTM gonads, whereas no thread-like SYCP3 signal was observed.
Our study demonstrates that aberrant development of gonad somatic cells leads to ectopic expression of meiosis-associated genes in germ cells, but meiosis was arrested before prophase I.
These results suggest that the proper differentiation of gonad somatic cells is essential for germ cell meiosis.
American Psychological Association (APA)
Chen, Min& Chen, Min& Chen, Suren& Zhou, Jingjing& Dong, Fangfang& Shen, Zhiming…[et al.]. 2019. Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1205404
Modern Language Association (MLA)
Chen, Min…[et al.]. Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1205404
American Medical Association (AMA)
Chen, Min& Chen, Min& Chen, Suren& Zhou, Jingjing& Dong, Fangfang& Shen, Zhiming…[et al.]. Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1205404
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205404