Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTPMPP+-Induced Toxicity via Progesterone Receptor Signaling
Joint Authors
Zhao, Yingke
Lee, Simon Ming-Yuen
Luo, Dan
Cheng, Yuanyuan
Rong, Jianhui
Zhao, Jia
Zhang, Xiuying
Lao, Lixing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Progesterone receptor (PR) modulates neuroprotective and regenerative responses in Parkinson’s disease and related neurological diseases.
Objectives.
The present study was designed to determine whether botanical drug puerarin could exhibit neuroprotective and neurorestorative activities via PR signaling.
Methods.
The neuroprotective and neurotrophic activities of puerarin were investigated in MPTP-lesioned mice and MPP+-challenged primary rat midbrain neurons.
Rotarod performance test and tail suspension test were used to assess motor functions.
Tyrosine hydroxylase (TH) and PR were determined by immunostaining, Western blotting, and luciferase reporter assays.
Neurite outgrowth was assessed by fluorescence staining and immunostaining.
Results.
Puerarin effectively ameliorated the MPTP-induced motor abnormalities in MPTP-lesioned mice and protected primary rat midbrain neurons against MPP+-induced toxicity via PR signaling although progesterone exhibited the neuroprotection.
PR antagonist mifepristone (RU486) diminished the neuroprotection of puerarin in MPTP-lesioned mice and MPP+-induced primary rat midbrain neurons.
Moreover, puerarin promoted the differentiation of primary rat midbrain neurons and potentiated NGF to induce neuritogenesis in PC12 cells.
RU486 and PR-siRNA could inhibit the effect of puerarin.
Puerarin and progesterone could enhance the PR promoter.
Conclusion.
Puerarin attenuated MPTP- and MPP+-induced toxicity and potentiated neurite outgrowth via PR.
These results suggested that puerarin may become an alternative hormone for suppressing MPTP- and MPP+-induced toxicity in neurodegenerative diseases.
American Psychological Association (APA)
Zhao, Yingke& Zhao, Jia& Zhang, Xiuying& Cheng, Yuanyuan& Luo, Dan& Lee, Simon Ming-Yuen…[et al.]. 2020. Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTPMPP+-Induced Toxicity via Progesterone Receptor Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205410
Modern Language Association (MLA)
Zhao, Yingke…[et al.]. Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTPMPP+-Induced Toxicity via Progesterone Receptor Signaling. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1205410
American Medical Association (AMA)
Zhao, Yingke& Zhao, Jia& Zhang, Xiuying& Cheng, Yuanyuan& Luo, Dan& Lee, Simon Ming-Yuen…[et al.]. Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTPMPP+-Induced Toxicity via Progesterone Receptor Signaling. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205410