Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers
Joint Authors
Lu, Han
Wang, Mi
Wang, Shanshan
Mao, Yanjun
Ying, Linlin
Li, Yao
Wang, Bo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-18
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
This study is aimed at evaluating the regulatory mechanism of quercetin on lipid metabolism in the ileum of broilers to better understand these pathways decreasing abdominal fat.
480 chickens were randomly divided into 4 groups (control, 0.02% quercetin, 0.04% quercetin, and 0.06% quercetin).
Breast muscle, thigh muscle, and abdominal fat pad were removed and weighed at 42 d of age.
Serum was obtained by centrifuging blood samples from the jugular vein (10 ml) to determine high-density lipoprotein (HDL), total cholesterol (TC), low-density lipoprotein (LDL), triglyceride (TG), leptin, and adiponectin using ELISA.
About 5 g of the ileum was harvested and immediately frozen in liquid nitrogen for RNA-seq.
Then, the confirmation of RNA-seq results by the Real-Time Quantitative PCR (RT-qPCR) method was evaluated using Pearson’s correlation.
Compared with control, abdominal fat percentage was significantly decreased with increasing quercetin supplementation, and the best result was obtained at 0.06% dietary quercetin supplementation (P<0.01).
Breast muscle percentage was significantly decreased at 0.02% quercetin (P<0.01), and thigh muscle percentage tended to increase (P=0.078).
Meanwhile, 0.04% and 0.06% quercetin significantly decreased TG (P<0.01), TC (P<0.01), and LDL content (P<0.05) in serum.
Serum leptin and adiponectin contents were significantly increased by 0.04% and 0.06% dietary quercetin supplementation, compared with the control (P<0.01).
Analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database were used to identify differently expressed genes and lipid metabolism pathways.
Quercetin decreased abdominal fat percentage through regulating fat digestion and absorption, glycerophospholipid metabolism, AMPK signaling pathway, fatty acid degradation, and cholesterol metabolism.
American Psychological Association (APA)
Wang, Mi& Mao, Yanjun& Wang, Bo& Wang, Shanshan& Lu, Han& Ying, Linlin…[et al.]. 2020. Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205675
Modern Language Association (MLA)
Wang, Mi…[et al.]. Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205675
American Medical Association (AMA)
Wang, Mi& Mao, Yanjun& Wang, Bo& Wang, Shanshan& Lu, Han& Ying, Linlin…[et al.]. Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205675
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205675