Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke
Joint Authors
Liu, Xinfeng
Xie, Yi
Liu, Qian
Zhang, Xiaohao
Shi, Xiaolei
Xu, Pengfei
Hong, Ye
Li, Juanji
Peng, Mengna
Xu, Gelin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Transplantation of neural stem cells (NSCs) is a promising therapy for ischemic stroke.
However, the effectiveness of this approach is limited by grafted cell death.
Breast cancer susceptibility protein 1 (BRCA1) could suppress apoptosis in neural progenitors and modulate oxidative stress in neurons.
In this study, we found that BRCA1 was upregulated by oxygen-glucose deprivation/reoxygenation (OGD/R).
Overexpression of BRCA1 in NSCs reduced cell apoptosis and oxidative stress after OGD/R insult.
The molecule overexpression also stimulated cellular proliferation in OGD/R NSCs and increased the survival rate of grafted cells.
Further, the transplantation of BRCA1-transfected NSCs into mice with ischemic stroke increased brain-derived neurotropic factor and nerve growth factor expression in the brain and elicited neurological function improvement.
In addition, we found that RING finger domain and BRCT domain of BRCA1 could physically interact with p53 in NSCs.
The cross talk between BRCA1 RING finger domain and p53 was responsible for p53 ubiquitination and degradation.
Our findings indicate that modification with BRCA1 could enhance the efficacy of NSCs transplantation in ischemic stroke.
American Psychological Association (APA)
Xu, Pengfei& Shi, Xiaolei& Zhang, Xiaohao& Liu, Qian& Xie, Yi& Hong, Ye…[et al.]. 2019. Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1205876
Modern Language Association (MLA)
Xu, Pengfei…[et al.]. Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1205876
American Medical Association (AMA)
Xu, Pengfei& Shi, Xiaolei& Zhang, Xiaohao& Liu, Qian& Xie, Yi& Hong, Ye…[et al.]. Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1205876
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205876