Mechanically Stretched Mesenchymal Stem Cells Can Reduce the Effects of LPS-Induced Injury on the Pulmonary Microvascular Endothelium Barrier
Joint Authors
Mao, Pu
Qiu, Haibo
Meng, Shan-shan
Xu, Xiu-Ping
Huang, Yong-bo
Li, Yi-min
Pan, Chun
Yang, Yi
Li, Jin-ze
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-30
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Mesenchymal stem cells (MSCs) may improve the treatment of acute respiratory distress syndrome (ARDS).
However, few studies have investigated the effects of mechanically stretched -MSCs (MS-MSCs) in in vitro models of ARDS.
The aim of this study was to evaluate the potential therapeutic effects of MS-MSCs on pulmonary microvascular endothelium barrier injuries induced by LPS.
We introduced a cocultured model of pulmonary microvascular endothelial cell (EC) and MSC medium obtained from MSCs with or without mechanical stretch.
We found that Wright-Giemsa staining revealed that MSC morphology changed significantly and cell plasma shrank separately after mechanical stretch.
Cell proliferation of the MS-MSC groups was much lower than the untreated MSC group; expression of cell surface markers did not change significantly.
Compared to the medium from untreated MSCs, inflammatory factors elevated statistically in the medium from MS-MSCs.
Moreover, the paracellular permeability of endothelial cells treated with LPS was restored with a medium from MS-MSCs, while LPS-induced EC apoptosis decreased.
In addition, protective effects on the remodeling of intercellular junctions were observed when compared to LPS-treated endothelial cells.
These data demonstrated that the MS-MSC groups had potential therapeutic effects on the LPS-treated ECs; these results might be useful in the treatment of ARDS.
American Psychological Association (APA)
Li, Jin-ze& Meng, Shan-shan& Xu, Xiu-Ping& Huang, Yong-bo& Mao, Pu& Li, Yi-min…[et al.]. 2020. Mechanically Stretched Mesenchymal Stem Cells Can Reduce the Effects of LPS-Induced Injury on the Pulmonary Microvascular Endothelium Barrier. Stem Cells International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1208003
Modern Language Association (MLA)
Li, Jin-ze…[et al.]. Mechanically Stretched Mesenchymal Stem Cells Can Reduce the Effects of LPS-Induced Injury on the Pulmonary Microvascular Endothelium Barrier. Stem Cells International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1208003
American Medical Association (AMA)
Li, Jin-ze& Meng, Shan-shan& Xu, Xiu-Ping& Huang, Yong-bo& Mao, Pu& Li, Yi-min…[et al.]. Mechanically Stretched Mesenchymal Stem Cells Can Reduce the Effects of LPS-Induced Injury on the Pulmonary Microvascular Endothelium Barrier. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1208003
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208003