Obesity-Induced Methylation of Osteopontin Contributes to Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells
Joint Authors
Tang, Min
Chen, Rui
Wang, Hao
Sun, Guowei
Yin, Fan
Liang, Beibei
Yang, Yang
Sharen, Gaowa
Wei, Huafeng
Zhou, Xuyu
Huang, Gang
Zhao, Jian
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-17
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Obesity is a major risk factor for many chronic diseases, including diabetes, fatty livers, and cancer.
Expansion of the adipose mass has been shown to be related to adipogenic differentiation of adipose-derived mesenchymal stem cells (ASCs).
However, the underlying mechanism of this effect has yet to be elucidated.
We found that osteopontin (OPN) is downregulated in ASCs and adipose tissues of obese mice and overweight human beings because of methylation on its promoter, indicating that OPN may affect the development of obesity.
Silencing of OPN in wild-type ASCs promotes adipogenic differentiation, while reexpression of OPN reduced adipogenic differentiation in OPN−/− ASCs.
The role of extracellular OPN in ASC differentiation was further demonstrated by supplementation and neutralization of OPN.
Additionally, OPN suppresses adipogenic differentiation in ASCs through the C/EBP pathways.
Consistent with these in vitro results, by intravenous injection of OPN-expressing adenovirus to the mice, we found OPN can delay the development of obesity and improve insulin sensitivity.
Therefore, our study demonstrates an important role of OPN in regulating the development of obesity, indicating OPN might be a novel target to attenuate obesity and its complications.
American Psychological Association (APA)
Tang, Min& Chen, Rui& Wang, Hao& Sun, Guowei& Yin, Fan& Liang, Beibei…[et al.]. 2019. Obesity-Induced Methylation of Osteopontin Contributes to Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208146
Modern Language Association (MLA)
Tang, Min…[et al.]. Obesity-Induced Methylation of Osteopontin Contributes to Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208146
American Medical Association (AMA)
Tang, Min& Chen, Rui& Wang, Hao& Sun, Guowei& Yin, Fan& Liang, Beibei…[et al.]. Obesity-Induced Methylation of Osteopontin Contributes to Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208146