Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors
Joint Authors
Huang, Yu
Sakai, Yusuke
Hara, Takanobu
Katsuda, Takeshi
Adachi, Tomohiko
Hidaka, Masaaki
Gu, Wei-Li
Eguchi, Susumu
Ochiya, Takahiro
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-03
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Chemically induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine.
Three-dimensional (3D) structures generated from liver progenitor cells possess wide applications in cell transplantation, disease model, and drug testing.
Here, we report on the spontaneous formation of 3D cystic structures comprising maturing rat CLiPs on gelatin-coated dishes.
Our 3D cysts contained Alb+/+CK19+/− and Ck19+/+Alb+/− cells.
These cell types gradually diverged into specialized mature cells, as demonstrated by the expression of mature biliary markers (Cftr, Ae2, and Aqp1) and hepatic markers (Alb and Mrp2).
The 3D cysts also expressed functional multidrug resistance protein 1 (Mdr1), as indicated by epithelial efflux of rhodamine.
Furthermore, we observed bile canaliculi functions between hepatocytes and cholyl-lysyl-fluorescein extrusions, indicating that the functional characteristics of 3D cysts and active bile salt export pump (Bsep) transporters were intact.
Thus, our study revealed a natural characteristic of rat CLiPs to spontaneously form 3D cystic structures accompanied with cell maturation in vitro, offering a platform for studies of liver development and drug screening.
American Psychological Association (APA)
Huang, Yu& Sakai, Yusuke& Hara, Takanobu& Katsuda, Takeshi& Ochiya, Takahiro& Adachi, Tomohiko…[et al.]. 2019. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
Modern Language Association (MLA)
Huang, Yu…[et al.]. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
American Medical Association (AMA)
Huang, Yu& Sakai, Yusuke& Hara, Takanobu& Katsuda, Takeshi& Ochiya, Takahiro& Adachi, Tomohiko…[et al.]. Development of Bifunctional Three-Dimensional Cysts from Chemically Induced Liver Progenitors. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208770