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Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches
Joint Authors
Varghese, Divya S.
Ansari, Suraiya A.
Alawathugoda, Thilina Thejinda
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-22
Country of Publication
Egypt
No. of Pages
18
Abstract EN
Human embryonic stem cells (hESCs) are being utilized in diverse areas of studies such as development and disease modeling, cell replacement therapy, or drug toxicity testing because of their potential to be differentiated into any cell type in the body.
The directed differentiation of hESCs into hepatocytes could provide an invaluable source of liver cells for various liver-based applications.
Therefore, several protocols have been established in the past for hESC-hepatocyte differentiation based on the knowledge of signaling pathways and growth factors involved in different stages of embryonic hepatogenesis.
Although successful derivation of hepatocytes has been achieved through these protocols, the efficiency is not always ideal.
Herein, we have tested several combinations of published protocols, for example, growth factor vs.
small molecule and different time durations of treatment for definitive endoderm (DE) induction and further hepatocyte differentiation to develop an efficient DE induction and hepatocyte differentiation in a highly reproducible manner based on the stage-specific marker expression and functional analysis.
American Psychological Association (APA)
Varghese, Divya S.& Alawathugoda, Thilina Thejinda& Ansari, Suraiya A.. 2019. Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches. Stem Cells International،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1209204
Modern Language Association (MLA)
Varghese, Divya S.…[et al.]. Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches. Stem Cells International No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1209204
American Medical Association (AMA)
Varghese, Divya S.& Alawathugoda, Thilina Thejinda& Ansari, Suraiya A.. Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1209204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209204