A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction
Joint Authors
Murray, Patricia
Sharkey, Jack
Wilm, Bettina
Ressel, Lorenzo
Brillant, Nathalie
Scarfe, Lauren
Park, B. Kevin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Cell-based regenerative medicine therapies require robust preclinical safety, efficacy, biodistribution, and engraftment data prior to clinical testing.
To address these challenges, we have developed an imaging toolbox comprising multispectral optoacoustic tomography and ultrasonography, which allows the degree of kidney, liver, and cardiac injury and the extent of functional recovery to be assessed noninvasively in a mouse model of multiorgan dysfunction.
This toolbox allowed us to determine the therapeutic effects of adoptively transferred macrophages.
Using bioluminescence imaging, we could then investigate the association between amelioration and biodistribution.
Macrophage therapy provided limited improvement of kidney and liver function, although not significantly so, without amelioration of histological damage.
No improvement in cardiac function was observed.
Biodistribution analysis showed that macrophages homed and persisted in the injured kidneys and liver but did not populate the heart.
Our data suggest that the limited improvement observed in kidney and liver function could be mediated by M2 macrophages.
More importantly, we demonstrate here the utility of the imaging toolbox for assessing the efficacy of potential regenerative medicine therapies in multiple organs.
American Psychological Association (APA)
Sharkey, Jack& Ressel, Lorenzo& Brillant, Nathalie& Scarfe, Lauren& Wilm, Bettina& Park, B. Kevin…[et al.]. 2019. A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209540
Modern Language Association (MLA)
Sharkey, Jack…[et al.]. A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209540
American Medical Association (AMA)
Sharkey, Jack& Ressel, Lorenzo& Brillant, Nathalie& Scarfe, Lauren& Wilm, Bettina& Park, B. Kevin…[et al.]. A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209540