Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production
Joint Authors
Huang, Bo
Gong, Ping
Tang, Hua
Yao, Qianqian
Zhang, Liang
Yao, Yang
He, Tao
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-09
Country of Publication
Egypt
No. of Pages
14
Abstract EN
This study is aimed at investigating the effect of amifostine (AMI) on rat bone marrow stromal stem cells (BMSCs) exposed to 2 Gy radiation.
The BMSCs were divided into four groups, namely, group A that received 0 Gy radiation, group B that received 0 Gy radiation and AMI, group C that received 2 Gy radiation, and group D that received 2 Gy radiation and AMI.
The proliferation, apoptosis, and distribution of BMSCs in the cell cycle, along with their osteogenesis ability, adipogenesis ability, and ROS production, were subsequently examined.
The levels of ALP, PPARγ, P53, and TNFα were determined by Western blotting.
The results demonstrated that the proliferation of BMSCs and the levels of ALP in group C were much lower than those in group A.
The production of ROS and levels of PPARγ, P53, and TNFα in the group that received 2 Gy radiation were much higher than those in group A.
Furthermore, the production of ROS and the levels of PPARγ, P53, and TNFα were much lower in group D than in group C.
Additionally, the levels of ALP and extent of cell proliferation were much higher in group D than in group C.
The results demonstrated the potential of AMI in reducing the side effects of radiation in BMSCs and in treatment of bone diseases caused by radiation.
American Psychological Association (APA)
Huang, Bo& He, Tao& Yao, Qianqian& Zhang, Liang& Tang, Hua& Gong, Ping…[et al.]. 2019. Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209830
Modern Language Association (MLA)
Tang, Hua…[et al.]. Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1209830
American Medical Association (AMA)
Huang, Bo& He, Tao& Yao, Qianqian& Zhang, Liang& Tang, Hua& Gong, Ping…[et al.]. Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209830