Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats
Joint Authors
Zhao, Liling
Wan, Xin-xing
Mo, Zhaohui
Wang, Fang
Yue, Chun
Wang, Linhao
Yang, Wenjun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-28
Country of Publication
Egypt
No. of Pages
7
Abstract EN
Objective.
To investigate the effects of mesenchymal stem cells (MSCs) coated by the extracellular matrix (ECM) on wound healing in diabetic rats.
Methods.
Mesenchymal stem cells were cocultured with ECM.
Cell viabilities were evaluated using MTT assay.
The diabetes model was established using both STZ and high-glucose/fat methods in SD rats.
A wound area was made on the middle of the rats’ back.
MSCs or ECM-MSCs were used to treat the rats.
HE staining and CD31 immunohistochemistry were used to detect the skin thickness and angiogenesis.
Western blotting and qRT-PCR were conducted to determine the level of VEGF-α, PDGF, and EGF.
Results.
It was observed that treatment of ECM had no significant effects on the cell viability of ECM-MSCs.
Wound area assay showed that both MSCs and ECM-MSCs could enhance the wound healing of diabetic rats and ECM-MSCs could further promote the effects.
Both MSCs and ECM-MSCs could enhance angiogenesis and epithelialization of the wounds, as well as the expression of VEGF-α, PDGF, and EGF in wound tissues, while ECM-MSC treatment showed more obvious effects.
Conclusion.
Mesenchymal stem cells coated by the extracellular matrix could promote wound healing in diabetic rats.
Our study may offer a novel therapeutic method for impaired diabetic wound healing.
American Psychological Association (APA)
Wang, Linhao& Wang, Fang& Zhao, Liling& Yang, Wenjun& Wan, Xin-xing& Yue, Chun…[et al.]. 2019. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats. Stem Cells International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1210018
Modern Language Association (MLA)
Wang, Linhao…[et al.]. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats. Stem Cells International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1210018
American Medical Association (AMA)
Wang, Linhao& Wang, Fang& Zhao, Liling& Yang, Wenjun& Wan, Xin-xing& Yue, Chun…[et al.]. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1210018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210018