Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells
Joint Authors
He, Fan
Chen, Xi
Yu, Jia
Ming, Pei
Lin, Jun
Pan, Guoqing
Wang, Yifan
Chen, Guangdong
Yan, Jinku
Zhu, Caihong
Zhang, Junxin
Yang, Huilin
Liu, Tao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Osteoarthritis is a chronic degenerative joint disease involving both articular cartilage and subchondral bone.
Kartogenin (KGN) was recently identified to improve in vivo cartilage repair; however, its effect on bone formation is unknown.
The aim of this study was to investigate the effect of KGN on antioxidant properties and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs).
Human BM-MSCs were treated with KGN at concentrations ranging from 10−8 M to 10−6 M.
Our results indicated that KGN improved cell proliferation and attenuated intracellular reactive oxygen species.
The levels of antioxidant enzymes and osteogenic differentiation of BM-MSCs were enhanced by KGN in a dose-dependent manner.
Furthermore, KGN-treated BM-MSCs showed upregulation of silent information regulator type 1 (SIRT1) and increased phosphorylation of adenosine 5′-monophosphate-activated protein kinase (AMPK), indicating that KGN activated the AMPK-SIRT1 signaling pathway in BM-MSCs.
Inhibition of SIRT1 by nicotinamide reversed the antioxidant effect of KGN on BM-MSCs and suppressed osteogenic differentiation.
In conclusion, our results demonstrated that KGN improved intracellular antioxidant properties and promoted osteogenic differentiation of BM-MSCs by activating the AMPK-SIRT1 signaling pathway.
Thus, KGN may have the potential for not only articular cartilage repair but also the clinical application of MSCs in bone tissue engineering.
American Psychological Association (APA)
Wang, Yifan& Chen, Guangdong& Yan, Jinku& Chen, Xi& He, Fan& Zhu, Caihong…[et al.]. 2018. Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1210870
Modern Language Association (MLA)
Wang, Yifan…[et al.]. Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1210870
American Medical Association (AMA)
Wang, Yifan& Chen, Guangdong& Yan, Jinku& Chen, Xi& He, Fan& Zhu, Caihong…[et al.]. Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1210870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210870