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Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyreneEthanol CoExposure
Joint Authors
Bucher, Simon
Le Guillou, Dounia
Allard, Julien
Pinon, Grégory
Begriche, Karima
Tête, Arnaud
Sergent, Odile
Lagadic-Gossmann, Dominique
Fromenty, Bernard
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-26
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Exposure to xenobiotics could favor the transition of nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis in obese patients.
Recently, we showed in different models of NAFL that benzo[a]pyrene (B[a]P) and ethanol coexposure induced a steatohepatitis-like state.
One model was HepaRG cells incubated with stearate and oleate for 2 weeks.
In the present study, we wished to determine in this model whether mitochondrial dysfunction and reactive oxygen species (ROS) overproduction could be involved in the occurrence of this steatohepatitis-like state.
CRISPR/Cas9-modified cells were also used to specify the role of aryl hydrocarbon receptor (AhR), which is potently activated by B[a]P.
Thus, nonsteatotic and steatotic HepaRG cells were treated with B[a]P, ethanol, or both molecules for 2 weeks.
B[a]P/ethanol coexposure reduced mitochondrial respiratory chain activity, mitochondrial respiration, and mitochondrial DNA levels and induced ROS overproduction in steatotic HepaRG cells.
These deleterious effects were less marked or absent in steatotic cells treated with B[a]P alone or ethanol alone and in nonsteatotic cells treated with B[a]P/ethanol.
Our study also disclosed that B[a]P/ethanol-induced impairment of mitochondrial respiration was dependent on AhR activation.
Hence, mitochondrial dysfunction and ROS generation could explain the occurrence of a steatohepatitis-like state in steatotic HepaRG cells exposed to B[a]P and ethanol.
American Psychological Association (APA)
Bucher, Simon& Le Guillou, Dounia& Allard, Julien& Pinon, Grégory& Begriche, Karima& Tête, Arnaud…[et al.]. 2018. Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyreneEthanol CoExposure. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1211428
Modern Language Association (MLA)
Bucher, Simon…[et al.]. Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyreneEthanol CoExposure. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-18.
https://search.emarefa.net/detail/BIM-1211428
American Medical Association (AMA)
Bucher, Simon& Le Guillou, Dounia& Allard, Julien& Pinon, Grégory& Begriche, Karima& Tête, Arnaud…[et al.]. Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyreneEthanol CoExposure. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1211428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211428