Protective Effect of Rosamultin against H2O2-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes
Joint Authors
Li, Jianyu
Zhang, Yongliang
Li, Lingzhi
Zhang, Ling
Liu, Yang
Gong, Hai Ying
Cui, Ying
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Rosamultin is one of the main active compounds isolated from Potentilla anserina L., which belongs to a triterpene compound.
Few studies have examined the effect of rosamultin on oxidative stress and its molecular mechanism.
The aim of this present study was to elucidate the protective effect of rosamultin on H2O2-induced oxidative damage and apoptosis in H9c2 cardiomyocytes and its mechanism.
The results showed that the pretreatment of rosamultin not only increased cell viability but also reduced the release of LDH and CK.
Rosamultin inhibited a H2O2-induced decrease in SOD, CAT, and GSH-Px activities and an increase in MDA content.
Meanwhile, ROS level, intracellular (Ca2+) fluorescence intensity, and apoptosis rate in the rosamultin pretreated group were markedly decreased compared with the model group.
Rosamultin pretreatment significantly reversed the morphological changes and attenuated H2O2-induced apoptosis.
Western blot analysis showed that rosamultin enhanced the expression of Bcl-2 and pCryAB and downregulated the expression of Bax, Cyt-c, Caspase-3, and Caspase-9 expression.
Additionally, rosamultin might activate PI3K/Akt signal pathways and CryAB relative factors.
Therefore, we suggest that rosamultin could have the potential for treating H2O2-induced oxidative stress injury through its antioxidant and antiapoptosis effect.
American Psychological Association (APA)
Zhang, Ling& Liu, Yang& Li, Jianyu& Li, Lingzhi& Zhang, Yongliang& Gong, Hai Ying…[et al.]. 2018. Protective Effect of Rosamultin against H2O2-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212217
Modern Language Association (MLA)
Zhang, Ling…[et al.]. Protective Effect of Rosamultin against H2O2-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1212217
American Medical Association (AMA)
Zhang, Ling& Liu, Yang& Li, Jianyu& Li, Lingzhi& Zhang, Yongliang& Gong, Hai Ying…[et al.]. Protective Effect of Rosamultin against H2O2-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212217
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212217