Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease
Joint Authors
Seo, Hyemyung
Son, Ilhong
Seol, Wongi
Ho, Dong Hwan
Jang, Jihoon
Lee, Sung Ik
Jeong, Soyeon
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Parkinson’s disease (PD) is the second most common neurodegenerative disease.
Oxidative stress is the most critical risk factor for neurodegenerative diseases, including Alzheimer’s disease (AD) and Huntington’s disease (HD).
Numerous reports have demonstrated that oxidative stress aggravates cytotoxicity in dopaminergic neurons and accelerates the formation of protein inclusions.
In addition, oxidative stress, such as 4-hydroxynonenal (HNE), oxidized protein, and dopamine quinone, are related to PD progression.
DJ-1 is a PD-causative gene, and it plays a pivotal role as a sensor and eliminator of oxidative stress.
Several studies have shown that oxidized DJ-1 (OxiDJ-1) formation is induced by oxidative stress.
Hence, previous studies suggest that oxidized DJ-1 could be a biomarker for PD.
We previously reported higher DJ-1 levels in Korean male PD patient urine exosomes than male non-PD controls.
We speculate that OxiDJ-1 levels in PD patient urine might be higher than that in non-PD controls.
In this study, we established an ELISA for OxiDJ-1 using recombinant DJ-1 treated with H2O2.
Using Western blot assay and ELISA, we confirmed an increase of OxiDJ-1 from HEK293T cells treated with H2O2.
Using our ELISA, we observed significantly higher, 2-fold, OxiDJ-1 levels in the urine of Korean PD patients than in non-PD controls.
American Psychological Association (APA)
Jang, Jihoon& Jeong, Soyeon& Lee, Sung Ik& Seol, Wongi& Seo, Hyemyung& Son, Ilhong…[et al.]. 2018. Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease. Parkinson’s Disease،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212443
Modern Language Association (MLA)
Jang, Jihoon…[et al.]. Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease. Parkinson’s Disease No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1212443
American Medical Association (AMA)
Jang, Jihoon& Jeong, Soyeon& Lee, Sung Ik& Seol, Wongi& Seo, Hyemyung& Son, Ilhong…[et al.]. Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease. Parkinson’s Disease. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212443
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212443